LOCUS       sequence-132516-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ampicillin resistant derivative of pDONR221 P1-P4 for multisite 
            GATEWAY cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Gu Y, Innes RW
  TITLE     The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED 
            DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome 
            vesicles.
  JOURNAL   Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. 
            Epub 2011 Feb 22.
  PUBMED    21343429
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.110.171785"; journalName: "Plant Physiol"; date: 
            "2011-04"; volume: "155"; issue: "4"; pages: "1827-38"
COMMENT     Sequence Label: pBSDONR P1-P4
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(480..711)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(761..778)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(761..777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(770..792)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
       61 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
      121 cgatacagta gaaattacag aaactttatc acgtttagta agtatagagg ctgaaaatcc
      181 agatgaagcc gaacgacttg taagagaaaa gtataagagt tgtgaaattg ttcttgatgc
      241 agatgatttt caggactatg acactagcgt atatgaatag gtagatgttt ttattttgtc
      301 acacaaaaaa gaggctcgca cctctttttc ttatttcttt ttatgattta atacggcatt
      361 gaggacaata gcgagtaggc tggatacgac gattccgttt gagaagaaca tttggaaggc
      421 tgtcggtcga ctaagttggc agcatcaccc gaagaacatt tggaaggctg tcggtcgact
      481 acaggtcact aataccatct aagtagttga ttcatagtga ctggatatgt tgtgttttac
      541 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      601 tttacgtttc tcgttcagct tttttgtaca aagttggcat tataaaaaag cattgctcat
      661 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      961 ttgttaaatc agctcatttt t
//
