LOCUS       sequence-132520-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation of NME1 gene disruption or mutation in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Schmitt ME, Clayton DA
  TITLE     Nuclear RNase MRP is required for correct processing of pre-5.8S 
            rRNA in Saccharomyces cerevisiae.
  JOURNAL   Mol Cell Biol. 1993 Dec;13(12):7935-41.
  PUBMED    8247008
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1993-12"; volume: "13(12)"; pages: "7935-41"
COMMENT     Sequence Label: pMES140
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     211..228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     212..228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     286..302
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     286..302
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
       61 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      121 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      181 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
      241 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
      301 tcccccgggc tgcaggaatt cctcgagcta cgtcagggct cttcaactaa agtttgagag
      361 ggcatttttt gttcgaacaa tatcagtgct aacaaggttg ctaccacatc aatgaactct
      421 actcctgtat gaaccctaat tttccttcat gaaaaatgaa ttttcgatgt ttttccgtgg
      481 attttatatt ttttcaaata tataaaggag taagaaaata actttgttta tcctatgttg
      541 cggtaaagaa ctaaatagtg gtcttcacct gttttctaat ttcttctcgt ttaaagttct
      601 tcaatccatg accaaagaat cgtcacaaat cgaagcttac aaaatggagt aaaatttttt
      661 ttactcagta atatgctttg ggttgaaagt ctcccaccaa ttcgtatgcg gaaaacgtaa
      721 tgagatttaa aaattttaaa ttgtttaaat caactcatta aggaggatgc ccttgggtat
      781 tctgcttctt gacctggtac ctctattgca gggtactggt gttttcttcg gtactggatt
      841 ccgtttgtat ggaatctaaa ccatagttat gacgattgct ctttcccgtg ctggatcgag
      901 taacccaatg gagcttacta ttcttggtcc atggattcac ccctttt
//
