LOCUS       sequence-132521-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation of NME1 gene disruption or mutation in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Schmitt ME, Clayton DA
  TITLE     Nuclear RNase MRP is required for correct processing of pre-5.8S 
            rRNA in Saccharomyces cerevisiae.
  JOURNAL   Mol Cell Biol. 1993 Dec;13(12):7935-41.
  PUBMED    8247008
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1993-12"; volume: "13(12)"; pages: "7935-41"
COMMENT     Sequence Label: pMES140
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    350..371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(386..391,392..409,410..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     429..451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     448..464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        485..503
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     531..547
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     532..548
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      481 tcgaaattaa ccctcactaa agggaacaaa agctggtacc gggccccccc tcgaggtcga
      541 cggtatcgat aagcttgata tcgaattcat tgtcacaata aggacgcata gttgttcatt
      601 ttagacatca tttacagcac ttccgtaaac taccccatat tgcgattgaa tactaccgaa
      661 gaggctcaag aatttacgcg gtgcccaatc aattatatag aatgaggaac aatggtaagt
      721 aatgaataat catggtcagt aataaaaaag caatctcgtc catgtttctt tttgaaactc
      781 agagcttgac tctgttgcat tggtatttca tcatttgatg aattattcac tattccatct
      841 tcttcttaca caagtttacc aataaataac tcatctgagt tattcagact ttttattaga
      901 tcagttcggc atatttaact atgcaaaagt tttacactgc taaaaa
//
