LOCUS       sequence-132527-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses high specificity SpCas9. Px330-like plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F
  TITLE     Rationally engineered Cas9 nucleases with improved specificity.
  JOURNAL   Science. 2015 Dec 1. pii: aad5227.
  PUBMED    26628643
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Dec 1. pii: aad5227."
COMMENT     Sequence Label: eSpCas9(1.1)
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
       61 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
      121 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
      181 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
      241 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      301 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      361 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      421 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      481 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      541 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
      601 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
      661 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
      721 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aacccc
//
