LOCUS       sequence-132528-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses high specificity SpCas9. Px330-like plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F
  TITLE     Rationally engineered Cas9 nucleases with improved specificity.
  JOURNAL   Science. 2015 Dec 1. pii: aad5227.
  PUBMED    26628643
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Dec 1. pii: aad5227."
COMMENT     Sequence Label: eSpCas9(1.1)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
       61 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
      121 gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg
      181 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
      241 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
      301 gtcgtgggaa ccgccctgat caaaaagtac cctgcgctgg aaagcgagtt cgtgtacggc
      361 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
      421 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
      481 ctggccaacg gcgagatccg gaaggcgcct ctgatcgaga caaacggcga aaccggggag
      541 atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa
      601 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg
      661 cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac
      721 ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
      781 ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga
      841 agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa
//
