LOCUS       sequence-132551-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To see S1 promoter activity of HBV for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Ko C, Lee S, Windisch MP, Ryu WS
  TITLE     DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis
            B virus replication at the transcriptional level.
  JOURNAL   J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 
            2014 Sep 17.
  PUBMED    25231298
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02035-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "23"; pages: "13689-98"
COMMENT     Sequence Label: pHBV-S1-Luc
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    203..294
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(235..254)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    308..356
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(624..645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc caagcttgtg ttgtttctct cttatataat atacccgcct tccatagagt
      121 gtgtaaatag tgtctagttt ggaagtaatg attaactact cgagcccggg ctagcacgcg
      181 taagagctcg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      241 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      301 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      361 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
      421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      481 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      781 ggagccccc
//
