LOCUS       sequence-132555-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), half of helix 4 truncated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1deltaH4/2
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             670..687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(740..758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      754..801
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(925..944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcatgaa aacctgtatt ttcagggcag cacctttagc aaactgcgtg
      121 aacagctggg cccggtgacc caggaatttt gggataacct ggaaaaagaa accgaaggcc
      181 tgcgtcagga aatgagcaaa gatctggaag aggtgaaagc gaaagtgcag ccgtatctgg
      241 atgactttca gaaaaaatgg cagccgctgc gtgcggaact gcaggaaggc gcgcgtcaga
      301 aactgcatga actgcaggaa aaactgagcc cgctgggcga agagatgcgt gatcgtgcgc
      361 gtgcgcatgt ggatgcgctg cgtacccatc tggcgccgta tagcgatgaa ctgcgtcagc
      421 gtctggcggc ccgtctggaa gcgctgaaag aaaacggcgg tgcgcgtctg gcggaatatc
      481 atgcgaaagc gaccgaacat ctgagcaccc tgagcgaaaa agcgaaaccg gcgctggaag
      541 atctgcgtca gggcctgctg ccggtgctgg aaagctttaa agtgagcttt ctgagcgcgc
      601 tggaagagta taccaaaaaa ctgaacaccc agtaagagct ccgtcgacaa gcttgcggcc
      661 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      781 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      961 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgattta
//
