LOCUS       sequence-132556-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), half of helix 4 truncated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1deltaH4/2
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(624..644)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(645..662)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             681..703
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    718..742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(742..761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(845..864)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcttac tgggtgttca gttttttggt atactcttcc
      121 agcgcgctca gaaagctcac tttaaagctt tccagcaccg gcagcaggcc ctgacgcaga
      181 tcttccagcg ccggtttcgc tttttcgctc agggtgctca gatgttcggt cgctttcgca
      241 tgatattccg ccagacgcgc accgccgttt tctttcagcg cttccagacg ggccgccaga
      301 cgctgacgca gttcatcgct atacggcgcc agatgggtac gcagcgcatc cacatgcgca
      361 cgcgcacgat cacgcatctc ttcgcccagc gggctcagtt tttcctgcag ttcatgcagt
      421 ttctgacgcg cgccttcctg cagttccgca cgcagcggct gccatttttt ctgaaagtca
      481 tccagatacg gctgcacttt cgctttcacc tcttccagat ctttgctcat ttcctgacgc
      541 aggccttcgg tttctttttc caggttatcc caaaattcct gggtcaccgg gcccagctgt
      601 tcacgcagtt tgctaaaggt gctgccctga aaatacaggt tttcatgatg atgatgatga
      661 tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt ctagagggga
      721 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      781 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      841 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      901 agcgcttgtt tcggcgtggg tatgg
//
