LOCUS       sequence-132557-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To see HBV S2 promoter activity for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Ko C, Lee S, Windisch MP, Ryu WS
  TITLE     DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis
            B virus replication at the transcriptional level.
  JOURNAL   J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 
            2014 Sep 17.
  PUBMED    25231298
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02035-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "23"; pages: "13689-98"
COMMENT     Sequence Label: pHBV-S2-Luc
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    328..419
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(360..379)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    433..481
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(749..770)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca ccacccaagc ttgtttctca aaggtggaga cagcggggta ggctgccttc
      121 ctgactggcg attggtggag gcaggaggcg gatttgctgg caaagtttgt agtatgccct
      181 gagcctgagg gctccacccc aaaaggcctc cgtgcggtgg ggtgaaaccc agcccgaatg
      241 ctccagctcc taccttgttg gcgtctggcc aggtgtcctt gttctcgagc ccgggctagc
      301 acgcgtaaga gctcggtacc tatcgataga gaaatgttct ggcacctgca cttgcactgg
      361 ggacagccta ttttgctagt ttgttttgtt tcgttttgtt ttgatggaga gcgtatgtta
      421 gtactatcga ttcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
      481 tgcggccgct ccaagtacct cccgtacctt aatattactt acttatcatg gtagcttggg
      541 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      601 tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      661 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      721 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      781 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      841 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      901 taaa
//
