LOCUS       sequence-132559-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  screen AB42 variants for aggregation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Wurth C, Guimard NK, Hecht MH
  TITLE     Mutations that reduce aggregation of the Alzheimer's Abeta42 
            peptide: an unbiased search for the sequence determinants of Abeta 
            amyloidogenesis.
  JOURNAL   J Mol Biol. 2002 Jun 21;319(5):1279-90.
  PUBMED    12079364
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2002-06-21"; volume: "319(5)"; pages: "1279-90"
COMMENT     Sequence Label: AB-GFP
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             317..1027
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(344..372)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     967..987
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1043..1060
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      121 ttttgtttaa ctttaagaag gagatataca tatggatgcg gaatttcgcc atgattctgg
      181 ctatgaagtg catcatcaga aactggtgtt ttttgcggaa gatgtgggct ctaacaaagg
      241 cgcgattatt ggcctgatgg tgggcggcgt ggtgattgcg ggatccgctg gctccgctgc
      301 tggttctggc gaattcagca aaggagaaga acttttcact ggagttgtcc caattcttgt
      361 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      421 tgctacatac ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc
      481 atggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga
      541 tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg
      601 cactatatct ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg
      661 tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat
      721 tctcggacac aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa
      781 acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggttccgt
      841 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      901 agacaaccat tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga
      961 ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct
     1021 ctacaaataa ggtaccctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
     1081 agcccgaaag gaagctga
//
