LOCUS       sequence-132561-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1080
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(13..336)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(351..377)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(378..398)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ataaagttgt gagacttttt tcttggcggc acaataaagt ttcctctttt tgttgggtca
       61 ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt tcgggtttaa
      121 agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga atcacatttt
      181 gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt cataaaataa
      241 taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga aagaagagtg
      301 atagagaaga agggaatgct tgaaaggatt ttgcatttat cactagttta ggcgtagtct
      361 gggacgtcgt atgggtagac cttacgcttc ttctttggtg gtccggatcc tccgtctcct
      421 ccgagttggg agaggtcgat acgggtctcg tagagtccgg tgatggattg gtggatgagg
      481 gtggcgtcga ggacctcctt ggtggaacgg tattgcttac ggtcgatggt ggtgtcgaag
      541 tacttgaagg cggctggggc tccgaggttg gtgagggtga agaggtggat gatgttctcg
      601 gcttgctcac ggattggctt gtcacggtgc ttgttgtagg cggagaggac cttgtcgagg
      661 ttggcgtcgg cgaggatgac acgcttggag aactcggaga tttgctcgat gatctcgtcg
      721 aggtagtgct tgtgttgctc gacgaagagt tgcttttgct cgttgtcctc tggggatccc
      781 ttgagcttct cgtagtggga ggcgaggtag aggaagttga cgtacttgga tgggagggcg
      841 agctcgtttc ccttttggag ctctccggcg gaggcgagca tacgcttacg tccgttctcg
      901 agctcgaaga gggagtactt tgggagcttg atgatgaggt ccttcttgac ctccttgtat
      961 cccttggcct c
//
