LOCUS       sequence-132565-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, chloramphenicol resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pOT2 3GLA
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    275..308
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      544..553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             565..1077
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     complement(604..625)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(865..884)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggatctcaag aagatccttt ggatcacaaa aaaaagcccg ctcattaggc gggttaaatt
       61 cccgggtact gcagccgatt cattaatgca ggttaacctg gcttatcgaa attaatacga
      121 ctcactatag ggagaccggc agatctgata tcatcgccac tgtgctggga attctgtcga
      181 cagctgcagt actagtcaag cttaggatcc gatatccatg gtgctagcga gatcttcctc
      241 gagggtaccg gcggccgcgt gcggccgcgt ctagataact tcgtataatg tatgctatac
      301 gaagttatca tgccggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa
      361 ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt
      421 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
      481 taagcaaata aacaagcgca gttgaacaag ctaaacaatc gggcggccgt actagagccg
      541 gtcgccacca tggtgtcgag cgcaagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      601 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      661 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      721 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      781 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      841 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      901 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      961 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1021 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1081 ggcagcgtgc agctcgccga
//
