LOCUS       sequence-132566-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, Amp resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pUC 3GLA
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    189..222
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      458..467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             479..937
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(518..539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(779..798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgcctt cttcttgaac tcgaattctg tcgacagctg cagtactagt caagcttagg
      121 atccgatatc catggtgcta gcgagatctt cctcgagggt accggcggcc gcgtgcggcc
      181 gcgtctagat aacttcgtat aatgtatgct atacgaagtt atcatgccgg ggatctaatt
      241 caattagaga ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga
      301 accctcgacc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      361 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagttgaa
      421 caagctaaac aatcgggcgg ccgtactaga gccggtcgcc accatggtgt cgagcgcaag
      481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      601 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      661 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      901 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      961 ggtgaacttc aagatc
//
