LOCUS       sequence-132567-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, Amp resistant, with 5xUAS and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pUC 3GLA UAS HAi
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    119..213
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    138..232
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        251..489
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     436..457
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             566..592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             602..628
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             608..634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    864..897
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgcctt cttcttgaac tcgaattgag gcgcgcccga agttatctag tagcaggtcg
      121 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      181 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact
      241 ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
      301 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
      361 aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa
      421 ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag
      481 agaactctga atagggaatt gggaattcga gatctaaaga gcctgctaaa gcaaaaaaga
      541 agtcaccatg tcgaccggct cgagctaccc ctacgacgtg cccgactacg cagctggtag
      601 ctacccctac gacgtgcccg actacgcatc gctagctaga tatcgcggta cctgtctaga
      661 cctgcaggat gcggccgcac actagtaggc ctgtagagtt tcccatttaa taattcatat
      721 tatctcgaat ctagtcaatt acggctttcc tcaaatagaa aaataaaaaa aatgaaaaaa
      781 tgcacttgcc atttaaactt agacgcgata acgttcaatt cgagttcaag aagaaggcgt
      841 ttttccatag gctccgcccc tagataactt cgtataatgt atgctatacg aagttatcat
      901 gccggggatc taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa
      961 gcttatcgat ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag
     1021 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     1081 acaagcgc
//
