LOCUS       sequence-132568-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, Amp resistant, with 5xUAS and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pUC 3GLA UAS HAi
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..48)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     376..397
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    586..619
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    819..888
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(989..1008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
       61 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      421 catggacgag ctgtacaagt actcagatct tgcaagcttg tagagtttcc catttaataa
      481 ttcatattat ctcgaatcta gtcaattacg gctttcctca aatagaaaaa taaaaaaaat
      541 gaaaaaatgc acttgccatt taaacttaga cgcgataagc ttgatataac ttcgtataat
      601 gtatgctata cgaagttata cgcgtactag atgcccgccg tgaccgtcga gaacccgctg
      661 acgctgcccc gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc
      721 accgcgccca gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc
      781 aactaccgcc acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg
      841 cccggggagc ccaagggcac gccctggcac ccgcaccgcg gcttcgagac ctgacctaca
      901 tcgtaccggc ttgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta
      961 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     1021 agccccgaca cccgc
//
