LOCUS       sequence-132569-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1083
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(15..338)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(353..379)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(380..400)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taataaagtt gtgagacttt tttcttggcg gcacaataaa gtttcctctt tttgttgggt
       61 cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc tttcgggttt
      121 aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca gaatcacatt
      181 ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa gtcataaaat
      241 aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa gaaagaagag
      301 tgatagagaa gaagggaatg cttgaaagga ttttgcattt atcactagtt taggcgtagt
      361 ctgggacgtc gtatgggtag accttacgct tcttctttgg tggtccggat cctccgtctc
      421 ctccgagttg ggagaggtcg atacgggtct cgtagagtcc ggtgatggat tggtggatga
      481 gggtggcgtc gaggacctcc ttggtggaac ggtactcctt acggtcgatg gtggtgtcga
      541 agtacttgaa ggcggctggg gctccgaggt tggtgagggt gaagaggtgg atgatgttct
      601 cggcttgctc acggattggc ttgtcacggt gcttgttgta ggcggagagg accttgtcga
      661 ggttggcgtc ggcgaggatg acacgcttgg agaactcgga gatttgctcg atgatctcgt
      721 cgaggtagtg cttgtgttgc tcgacgaaga gttgcttttg ctcgttgtcc tctggggatc
      781 ccttgagctt ctcgtagtgg gaggcgaggt agaggaagtt gacgtacttg gatgggaggg
      841 cgagctcgtt tcccttttgg agctcacggg cggaggcgag catacgctta cgtccgttct
      901 cgagctcgaa gagggagtac tttgggagct tgatgatgag gtccttcttg acctccttgt
      961 atcccttggc ctcgaggaag tcgattgggt tcttctcgaa ggaggaacgc tccatgatgg
     1021 tgattccgag gagctccttg acggacttga gcttcttgga ctttcccttc tcgaccttgg
     1081 cgacgacgag gacggagtag gcgacggttg ggga
//
