LOCUS       sequence-132570-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1083
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(325..924)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(925..945)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(965..981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(965..981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcctcgagac ggtggaagaa ggagtcgtcg accttggcca tctcgttgga gaagatctct
       61 tggaggtagc agatacggtt cttacgacgg gtgtaacgac gacgggcggt acgcttgaga
      121 cgggtggcct cggcggtctc tccggagtcg aagaggaggg ctccgatgag gttcttcttg
      181 atggagtgac ggtcggtgtt tccgaggacc ttgaacttct tggatgggac cttgtactcg
      241 tcggtgatga cggcccatcc gacggagttc gttccgatgt caaggccgat gctgtatttt
      301 ttgtccattt tttctagagc aaagtgtttc ccaactgaaa aaaaaacaat ttaatttaaa
      361 agtaagaaga gtgcggacgg tagagagaat aaaagtcgaa aaaattatcc tagaaacttc
      421 taatattttt aaaaattaaa aaaatataaa aagtgaggtc acaaataaga cagttagaac
      481 atttgttgaa agcctaaaat aaaaattcga ctaaacagtg agaacaggaa aaacgctggg
      541 actgaatgtt tgacctcatt ataaattcat aaaatttaaa tttcctaatc tggataaaaa
      601 caaagataaa aacaaaataa accaacaaaa aaccaacatg attagtcaga tgaccagaaa
      661 actcacgtta atttcacaag gcccaaaaaa atctctcccc ctctcgttgc tgcctgcaca
      721 tctaactcct agcacgaaaa tgtaccgtac accatttcaa acactctcgg cgggagtgtt
      781 gcatactttt tctctctggc agtttatttt caataaattc ttcattttta tattgtataa
      841 tgtctttatt ttatgaaaaa ttcattaatt taacagaaac aatggaagaa ccaatggaag
      901 ttgacaataa aagaccaaag gtgccaactt ttctatacaa agttgatagc ttggcgtaat
      961 catggtcata gctgtttcct gtgtgaaatt gttatccgc
//
