LOCUS       sequence-132571-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 
            variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1081
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      104..131
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(143..730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(223..242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     770..787
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     771..787
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga
       61 tgctcgatga gtttttctaa tgaggaccta aatgtaatca cctggctcac cttcgggtgg
      121 gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      181 atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      601 gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca
      661 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      721 aggatctcaa gaagatcctt tgattttcta ccgaagaaag gcccacccgt gtaaaacgac
      781 ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt gagccagtga
      841 gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata tgcgaccgcc
      901 ggagggttgc gtttgagacg ggcgacagat ccagtcgcgc tgctctcgtc gatccaagct
      961 tcaaaaa
//
