LOCUS       sequence-132572-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 
            variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1081
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(58..160)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(161..192)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     240..256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(399..474)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 catgttggaa tttaatcgcg gcttcaagca agacgtttcc cgttgaatat ggctcatttt
       61 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      121 ttcattatgg tgaaagttgg aacctcttac gtgccgatca agtcaaaagc ctccggtcgg
      181 aggcttttga ctttctgcta tggaggtcag gtatgattta aatggtcagt attgagcctc
      241 aggaaacagc tatgacatca agctgactag ataatctagc tgatcgtgga ccgatcatac
      301 gtataatgcc gtaagatcac gggtcgcagc acagctcgcg gtccagtagt gatcgacact
      361 gctcgatccg ctcgcaccgc tagcaaattt cacaaaaagc accgactcgg tgccactttt
      421 tcaagttgat aacggactag ccttatttta acttgctatt tctagctcta aaactggtgc
      481 ctatggtagc ggacaagaca tctcgcaata ggaggtgttt atataggggc aacacaagag
      541 gcggcagaca tctcgtgagt ttgacagagt agtataaaga gtgtgtgtga gagggtgtag
      601 gcatttcata acatattttt tgtggaataa ttttggaaaa aaggtgtttt tttaataata
      661 gcttttataa gagtttctca aaaattgcat gctttttttg aaaaggttga caaattatag
      721 tagtactaaa gttgctattt tcaagtctca gcagaaaatt cacaactaaa aataaaattt
      781 gcaaaacaga gtgtgcgcct ttaaggtgta cagtttttta ttcctttatt gctagttttt
//
