LOCUS       sequence-132574-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1084
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      106..133
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(145..732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(225..244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     772..789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     773..789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcactt
       61 gatgctcgat gagtttttct aatgaggacc taaatgtaat cacctggctc accttcgggt
      121 gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      181 aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      601 ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa
      661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      721 aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgtaaaacg
      781 acggccagtt tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt
      841 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcgaccg
      901 ccggagggtt gcgtttgaga cgggcgacag atccagtcgc gctgctctcg tcgatccaag
      961 cttcaaaaa
//
