LOCUS       sequence-132580-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PICK1 as well as the marker Venus 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pVenus-PICK1-shRNA
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(101..120)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             260..511
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     448..469
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    552..686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(576..595)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     630..649
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
       61 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      121 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      181 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      241 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      301 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      361 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
      421 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      481 gatcactctc ggcatggacg agctgtacaa gtaagtcgag cctctagaac tatagtgagt
      541 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
      601 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      661 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      721 ttcaggggga ggtgtgggag gttttta
//
