LOCUS       sequence-132588-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      39..1020
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt
       61 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
      121 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
      181 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
      241 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
      301 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
      361 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
      421 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
      481 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
      541 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
      601 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
      661 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
      721 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
      781 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
      841 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
      901 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
      961 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
//
