LOCUS       sequence-132590-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the catalytic domain of human SENP3. Suitable for in vitro 
            transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP3(307-574)
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             908..925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatgagcccc ctgcgagagg agcatgtgac
      121 ctgcgtacag agcatcttgg acgaattcct tcaaacgtat ggcagcctca tacccctcag
      181 cactgatgag gtagtagaga agctggagga cattttccag caggagtttt ccaccccttc
      241 caggaagggc ctggtgttgc agctgatcca gtcttaccag cggatgccag gcaatgccat
      301 ggtgaggggc ttccgagtgg cttataagcg gcacgtgctg accatggatg acttggggac
      361 cttgtatgga cagaactggc tcaatgacca ggtgatgaac atgtatggag acctggtcat
      421 ggacacagtc cctgaaaagg tgcatttctt caatagtttc ttctatgata aactccgtac
      481 caagggttat gatggggtga aaaggtggac caaaaacgtg gacatcttca ataaggagct
      541 actgctaatc cccatccacc tggaggtgca ttggtccctc atctctgttg atgtgaggcg
      601 acgcaccatc acctattttg actcgcagcg taccctaaac cgccgctgcc ctaagcatat
      661 tgccaagtat ctacaggcag aggcggtaaa gaaagaccga ctggatttcc accagggctg
      721 gaaaggttac ttcaaaatga atgtggccag gcagaataat gacagtgact gtggtgcttt
      781 tgtgttgcag tactgcaagc atctggccct gtctcagcca ttcagcttca cccagcagga
      841 catgcccaaa cttcgtcggc agatctacaa ggagctgtgt cactgcaaac tcactgtgtg
      901 actcgagcac caccaccacc accactga
//
