LOCUS       sequence-132607-        4084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK567
FEATURES             Location/Qualifiers
     source          1..4084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        517..535
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    536..560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             669..713
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(751..769)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      765..812
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(983..1774)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1775..1866)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1914..2652)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(2862..3944)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3906..3925
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3945..4022)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(4068..4084,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggcaaagc gcacccttct tatagcgctg gcattggcat gcgcggcacc
      121 gggcttggcc catgcccagc gcattgcgcc catgaaggca ggtaactttg cccgcctttg
      181 tacgcgcgcc actagtgctg gcatttgcga tgcctatatt ggcggcctga cggatggggt
      241 ctctctttcc aaaatcaatg atcggaacga gggggatcct tctgcgcctg ctggtttttg
      301 tgttcccgct acagaaacca tgcccgttat gcgctccaag gtacttacct ggctgaaagc
      361 gcattctgaa acactggaca agccggttgg tgaaagtgtt tttgccgccc tgcacgcctc
      421 ttatccgtgc agtgtgaaaa aatgaaagct tgcggccgca taatgcttaa gtcgaacaga
      481 aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat
      541 tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac
      601 atatggcaga tctcaattgg atatcggccg gccacgcgat cgctgacgtc ggtaccctcg
      661 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
      721 gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg
      781 gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca
      841 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
      901 tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc
      961 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     1021 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     1081 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     1141 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     1201 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     1261 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     1321 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     1381 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     1441 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     1501 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     1561 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     1621 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     1681 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     1741 gagtcgatac ttcggcgatc accgcttccc tcatactctt cctttttcaa tattattgaa
     1801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1861 aacaaatagc tagctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc
     1921 ggacacatac aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca
     1981 aaacagcagg gccgcgccgg tggcgttttt ccataggctc cgccctcctg ccagagttca
     2041 cataaacaga cgcttttccg gtgcatctgt gggagccgtg aggctcaacc atgaatctga
     2101 cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggcg ggtcgctccc
     2161 tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgttccgc ctttctccct
     2221 tacgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc ggtgtaggtc
     2281 gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta
     2341 tccggtaact gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc actggcagca
     2401 gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga
     2461 agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa
     2521 gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc
     2581 caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa
     2641 gatcctttga tcttttctac tgaaccgctc tagatttcag tgcaatttat ctcttcaaat
     2701 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc
     2761 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2821 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2881 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2941 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     3001 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     3061 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     3121 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
     3181 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     3241 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     3301 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     3361 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     3421 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     3481 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     3541 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     3601 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3661 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3721 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3781 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3841 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3901 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3961 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     4021 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa tacgactcac
     4081 tata
//
