LOCUS       sequence-132608-        7100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase subunits, one with C-terminal His6 tag, and associated 
            hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK598
FEATURES             Location/Qualifiers
     source          1..7100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2472..2491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2632..2654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3348..3367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3348..3366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3398..3420
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6944..6961
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7014..7032)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7028..7075
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
     3361 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
     3421 tatacatatg aaggtacgtg taaccgtcat gctgaaggac ggtgttctgg acccgcaggg
     3481 caaggccatc agccacgcat tgcagacact tggttttgaa ggcgtgcacg atgtgcgcgt
     3541 gggtaaggtg attgaactgg atctgccggg tgccaaagcc gaggaagccc agaaaaaggc
     3601 cgatgccatg gcgcgtgatc tgctagccaa cctggtgatt gaagattact ctgtagaggt
     3661 ggtggaatga agcgcaccct tcttatagcg ctggcattgg catgcgcggc accgggcttg
     3721 gcccatgccc agcgcattgc gcccatgaag gcaggtaact ttgcccgcct ttgtacgcgc
     3781 gccactagtg ctggcatttg cgatgcctat attggcggcc tgacggatgg ggtctctctt
     3841 tccaaaatca atgatcggaa cgagggggat ccttctgcgc ctgctggttt ttgtgttccc
     3901 gctacagaaa ccatgcccgt tatgcgctcc aaggtactta cctggctgaa agcgcattct
     3961 gaaacactgg acaagccggt tggtgaaagt gtttttgccg ccctgcacgc ctcttatccg
     4021 tgcagtgtga aaaaatgaaa gcagcaatta tcgtattccc cggtaccaac cgggagcgcg
     4081 atatggccat tgccctcaag cgcgttacag ggcaggcccc gcgcatgatc tggcaccatg
     4141 aaacagatct gggagatacc gatctagtcg tgctgccagg tggtttcagc tatggcgatt
     4201 atctgcgctg tggtgccatg gcagcgcatt ctcccatcat ggcggctatc aaagcctttg
     4261 cccaaaaggg tggccatatt ttgggtgtgt gcaacggctt ccagatttta acggaaacag
     4321 ggcttctgcc cggtgctctg ttgcgcaacg ccaacctgcg ctttctctct caggattgct
     4381 acctgaaggt ggaagcagag aacacacctt tcactcacaa gtggaagaag ggtgatgttt
     4441 tccgcacccc tctggcccat ggtgatggca attacacggc agatatcgcc acgctggaac
     4501 ggcttgaagg tgaaggtctt gtggctttcc actatgccga tgccaaaggc aatgtgcagg
     4561 ctgatgatgc catcagcaac cccaatggca gcatcaacag cattgcgggt attctgagcg
     4621 aaaacaaacg catttgcggc atgatgcctc atccagaaga tctggttgat cctctcatgg
     4681 gtggggaaga tggtctgcca ttgttccaag gtctggtgga ggctgtagtc cggtgaataa
     4741 gcctgtaacc gttgatgaat cccttgcccg tgaattcggg ctgacatctg aggaatacgg
     4801 tcgtgtgctg tccattatgg gccgcacccc cagcctgaca gagctgggcg tgttctctgt
     4861 gatgtggtcg gaacactgct cctacaaatc ttcccgcaag tggctgcgca cgctgccaac
     4921 caaggcgccg tgggttattc atggccccgg tgaaaatgct ggtgtggtgg atattgggca
     4981 gggctatgcc gccatcttca agatggaaag ccataaccac ccatccttta tcgaacccta
     5041 tcagggtgcg gccacgggtg tggggggcat tctgcgcgat gtgttcacca tgggcgcacg
     5101 ccccattgcc aacttgaacg ccctgcgttt tggcagcccc gaaaaccccg ttacccgccg
     5161 cgtgattgat ggcgtggtgc gtggtattgg cggctatggc aactgtgttg gcgtgcccac
     5221 cgtaggcggg gaaatcaact tccacccggc ctatgatggc aacccgctgg taaacgccat
     5281 gacggttggt ttggcccgta aggaccgtat cttcctttcg gctgcggcag gtgttggcaa
     5341 cccggttgtt tacgtgggct cccgcacagg gcgtgatggc attcatggtg ccaccatgtc
     5401 ttccgccgag tttgatgaag atgccctttc caagcgcccc acggtacagg tgggtgaccc
     5461 ctttattgaa aagcttctga ttgaagcctg tctggaactg atggcaacag atgccattat
     5521 cgccattcag gacatgggcg ctgcgggtct gacatcttct tccgtggaaa tggcaggcaa
     5581 gggcggtgtg ggcattgagc ttgatctgga tgccgtgccc cagcgtgagc cgaacatgac
     5641 ggcttatgaa atgatgctgt ctgaaagcca ggaacgcatg ctgatggtgc tgcgccctga
     5701 ccggacagac gtggcccgca agatctttga aaaatgggaa ctggattttg ccattattgg
     5761 gcatctgaca gataccggcc acatcgtcgt caagcacaaa ggacagttgg aagccgatat
     5821 tccgctggac ccattggcag aacaggcccc tatttacgat cggcccgcaa caccacccaa
     5881 ggcgccggct ccgctagaag gcgtgcatgc accgctttct ttggatgcca cgttactgaa
     5941 gctggttggt tcgccagatc tggcctcccg cgcatgggta tggaaccagt atgacagcac
     6001 tgtagggggg cagacagtcc gccgtccggg tgctgctgat gctgccattg tgaaggtgga
     6061 agatacagac attggtctgg ccatcaccac agattgcacc ccgcgttact gtcaggcaga
     6121 ccccaaaaca ggtggcgcac aagctgttgc agaagcatgg cgtaacctaa cagctactgg
     6181 cgcacgcccg ctggccgtaa cggataacct gaacttcgcc aacccggaaa aacccgaaat
     6241 catgggccag tttgtggctg cggttgaagg tatgggggaa gcctgtacag cgctggattt
     6301 cccggtcgtg agcggcaacg tgtctcttta taatgaaaca cgaaacccag atggctccac
     6361 caccgccatt ctgcccacac ccgccattgg cgccttgggc gttctggatg atgtgaacaa
     6421 ggctgttggc cttggcatgc agccagaatc tgacgtggtg ctgattggtg aaaccaaggg
     6481 tgagctcggc cagaccatct ggctgcgtga aattctgggt caggaagctg gtgcaccgcc
     6541 gccggtcgat ctggttgccg aaaaacgcaa tggtgacttt gtacgcagcg ccattctgga
     6601 tggcaccatt accgcatgcc atgacattgc tgacggtggc attatggttg ccgccgcaga
     6661 aatggtgatg gcaagtggca ttggatgtga gctggaaagc ccagattccg gtatccggcc
     6721 agaagccttc tggtttggtg aagatcaggc ccgctatctg gcttgtgtag caaacggcaa
     6781 agccctgtgc gaacgcgctc aggcagctgg tgtgcctgca cgcattattg cccgctcagg
     6841 cggaaatgat ttgattctgc ccagcggcgt caccatatcc gcagcacgcc taaaaacggc
     6901 ccatgaagca tttttcccga tgctgatggg caactcactc gagcaccacc accaccacca
     6961 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     7021 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     7081 aggaggaact atatccggat
//
