LOCUS       sequence-132614-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human VCPIP (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-VCPIP (OTU, aa 25-561)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gtttaaatgg tctagaaagc tttagtctcc
       61 atccaaatac acaatagacc catctcctct gacctttcgc acagatgtgc catggcacca
      121 attacaagct gttaggttac tcattccata gtctggtacc agaacatctt tcactgaatc
      181 atatgagcaa accaaattgt tcaagggaaa gctgtaattt ttgtcagtcc tcagctgtcc
      241 atgagtactt tttgccaggt tatacaattt ccctccagga gccaaccact ctggaggaac
      301 atgaagttca gaaagggcac cacagagcaa acatttgtga aggcgattat ccattactgc
      361 ttttttagca gctgctgtga cttcctcagg ctgaactcct atcactccag tccttctgta
      421 gaaatactga tggacatcag caaccaaact aggatggata ccatgtttgt ccataaagac
      481 ttcttccata gcagcaacaa gcctaagtaa gtatttatct tgcaaacttc tgtcacctcc
      541 aataacacaa ccaccatcct cttcaagttt tatgtacttt ttaataaggt cctgaggcac
      601 accccatgct ttaggaagca aattcatagg cagtttgggc aaagcagccc cttttatgcc
      661 taccaagggg atataatggt ttctaccgga gctgctccat gcaatacaga ttggtttgtt
      721 caaatgacca tctttcccag tgcacttctc tgcagggatg agcccaggta gaaaggtggc
      781 tgaataatca ccagagcttc tcatgccact gagggaatct aacagaataa taggacgatg
      841 tagcacattg gcaagaccaa atatgtggat attcctcagg cccaagggaa caccctcagg
      901 tggtacaaac agagggtcac actcattgat aatgtcctc
//
