LOCUS       sequence-132617-        6732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); 
            large subunit with C-terminal His6 tag (AaPurLH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK606
FEATURES             Location/Qualifiers
     source          1..6732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3313..3335
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3366..3385)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3367..3385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3894..4085
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4079..4101)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4187..4329
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4242..4261
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4344..4361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4515..5103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4595..4614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5274..6065,6066..6134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5897..5916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6135..6239)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6266..6721)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6403..6424)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6615..6634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttagttg cccatcagca
      181 tcgggaaaaa tgcttcatgg gccgttttta ggcgtgctgc ggatatggtg acgccgctgg
      241 gcagaatcaa atcatttccg cctgagcggg caataatgcg tgcaggcaca ccagctgcct
      301 gagcgcgttc gcacagggct ttgccgtttg ctacacaagc cagatagcgg gcctgatctt
      361 caccaaacca gaaggcttct ggccggatac cggaatctgg gctttccagc tcacatccaa
      421 tgccacttgc catcaccatt tctgcggcgg caaccataat gccaccgtca gcaatgtcat
      481 ggcatgcggt aatggtgcca tccagaatgg cgctgcgtac aaagtcacca ttgcgttttt
      541 cggcaaccag atcgaccggc ggcggtgcac cagcttcctg acccagaatt tcacgcagcc
      601 agatggtctg gccgagctca cccttggttt caccaatcag caccacgtca gattctggct
      661 gcatgccaag gccaacagcc ttgttcacat catccagaac gcccaaggcg ccaatggcgg
      721 gtgtgggcag aatggcggtg gtggagccat ctgggtttcg tgtttcatta taaagagaca
      781 cgttgccgct cacgaccggg aaatccagcg ctgtacaggc ttcccccata ccttcaaccg
      841 cagccacaaa ctggcccatg atttcgggtt tttccgggtt ggcgaagttc aggttatccg
      901 ttacggccag cgggcgtgcg ccagtagctg ttaggttacg ccatgcttct gcaacagctt
      961 gtgcgccacc tgttttgggg tctgcctgac agtaacgcgg ggtgcaatct gtggtgatgg
     1021 ccagaccaat gtctgtatct tccaccttca caatggcagc atcagcagca cccggacggc
     1081 ggactgtctg cccccctaca gtgctgtcat actggttcca tacccatgcg cgggaggcca
     1141 gatctggcga accaaccagc ttcagtaacg tggcatccaa agaaagcggt gcatgcacgc
     1201 cttctagcgg agccggcgcc ttgggtggtg ttgcgggccg atcgtaaata ggggcctgtt
     1261 ctgccaatgg gtccagcgga atatcggctt ccaactgtcc tttgtgcttg acgacgatgt
     1321 ggccggtatc tgtcagatgc ccaataatgg caaaatccag ttcccatttt tcaaagatct
     1381 tgcgggccac gtctgtccgg tcagggcgca gcaccatcag catgcgttcc tggctttcag
     1441 acagcatcat ttcataagcc gtcatgttcg gctcacgctg gggcacggca tccagatcaa
     1501 gctcaatgcc cacaccgccc ttgcctgcca tttccacgga agaagatgtc agacccgcag
     1561 cgcccatgtc ctgaatggcg ataatggcat ctgttgccat cagttccaga caggcttcaa
     1621 tcagaagctt ttcaataaag gggtcaccca cctgtaccgt ggggcgcttg gaaagggcat
     1681 cttcatcaaa ctcggcggaa gacatggtgg caccatgaat gccatcacgc cctgtgcggg
     1741 agcccacgta aacaaccggg ttgccaacac ctgccgcagc cgaaaggaag atacggtcct
     1801 tacgggccaa accaaccgtc atggcgttta ccagcgggtt gccatcatag gccgggtgga
     1861 agttgatttc cccgcctacg gtgggcacgc caacacagtt gccatagccg ccaataccac
     1921 gcaccacgcc atcaatcacg cggcgggtaa cggggttttc ggggctgcca aaacgcaggg
     1981 cgttcaagtt ggcaatgggg cgtgcgccca tggtgaacac atcgcgcaga atgcccccca
     2041 cacccgtggc cgcaccctga tagggttcga taaaggatgg gtggttatgg ctttccatct
     2101 tgaagatggc ggcatagccc tgcccaatat ccaccacacc agcattttca ccggggccat
     2161 gaataaccca cggcgccttg gttggcagcg tgcgcagcca cttgcgggaa gatttgtagg
     2221 agcagtgttc cgaccacatc acagagaaca cgcccagctc tgtcaggctg ggggtgcggc
     2281 ccataatgga cagcacacga ccgtattcct cagatgtcag cccgaattca cgggcaaggg
     2341 attcatcaac ggttacaggc ttattcaccg gactacagcc tccaccagac cttggaacaa
     2401 tggcagacca tcttccccac ccatgagagg atcaaccaga tcttctggat gaggcatcat
     2461 gccgcaaatg cgtttgtttt cgctcagaat acccgcaatg ctgttgatgc tgccattggg
     2521 gttgctgatg gcatcatcag cctgcacatt gcctttggca tcggcatagt ggaaagccac
     2581 aagaccttca ccttcaagcc gttccagcgt ggcgatatct gccgtgtaat tgccatcacc
     2641 atgggccaga ggggtgcgga aaacatcacc cttcttccac ttgtgagtga aaggtgtgtt
     2701 ctctgcttcc accttcaggt agcaatcctg agagagaaag cgcaggttgg cgttgcgcaa
     2761 cagagcaccg ggcagaagcc ctgtttccgt taaaatctgg aagccgttgc acacacccaa
     2821 aatatggcca cccttttggg caaaggcttt gatagccgcc atgatgggag aatgcgctgc
     2881 catggcacca cagcgcagat aatcgccata gctgaaacca cctggcagca cgactagatc
     2941 ggtatctccc agatctgttt catggtgcca gatcatgcgc ggggcctgcc ctgtaacgcg
     3001 cttgagggca atggccatat cgcgctcccg gttggtaccg gggaatacga taattgctgc
     3061 tttcattcca ccacctctac agagtaatct tcaatcacca ggttggctag cagatcacgc
     3121 gccatggcat cggccttttt ctgggcttcc tcggctttgg cacccggcag atccagttca
     3181 atcaccttac ccacgcgcac atcgtgcacg ccttcaaaac caagtgtctg caatgcgtgg
     3241 ctgatggcct tgccctgcgg gtccagaaca ccgtccttca gcatgacggt tacacgtacc
     3301 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
     3361 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcgggc agcgttgggt
     3421 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     3481 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     3541 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     3601 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
     3661 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
     3721 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
     3781 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
     3841 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca
     3901 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc
     3961 tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg
     4021 accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     4081 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4141 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     4201 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     4261 actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4321 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4501 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4681 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4921 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5101 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5161 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5221 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5281 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5341 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5401 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5461 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5521 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5581 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5641 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5701 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5761 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5821 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5881 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5941 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6001 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6061 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6121 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6181 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6241 acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt aaaattcgcg
     6301 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     6361 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     6421 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     6481 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     6541 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     6601 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     6661 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     6721 tcccattcgc ca
//
