LOCUS       sequence-132621-        6938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase subunits; associated hypothetical protein N-terminal 
            domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK612
FEATURES             Location/Qualifiers
     source          1..6938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2472..2491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2632..2654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3348..3367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3348..3366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3398..3420
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6782..6799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6852..6870)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6866..6913
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
     3361 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
     3421 tatacatatg aaggtacgtg taaccgtcat gctgaaggac ggtgttctgg acccgcaggg
     3481 caaggccatc agccacgcat tgcagacact tggttttgaa ggcgtgcacg atgtgcgcgt
     3541 gggtaaggtg attgaactgg atctgccggg tgccaaagcc gaggaagccc agaaaaaggc
     3601 cgatgccatg gcgcgtgatc tgctagccaa cctggtgatt gaagattact ctgtagaggt
     3661 ggtggaatga agcgcaccct tcttatagcg ctggcattgg catgcgcggc accgggcttg
     3721 gcccatgccc agcgcattgc gcccatgaag gcaggtaact ttgcccgcct ttgtacgcgc
     3781 gccactagtg ctggcatttg cgatgcctat attggcggcc tgacggatgg ggtctctctt
     3841 tccaaaatca atgatcggaa cgagggggat ccatgaaagc agcaattatc gtattccccg
     3901 gtaccaaccg ggagcgcgat atggccattg ccctcaagcg cgttacaggg caggccccgc
     3961 gcatgatctg gcaccatgaa acagatctgg gagataccga tctagtcgtg ctgccaggtg
     4021 gtttcagcta tggcgattat ctgcgctgtg gtgccatggc agcgcattct cccatcatgg
     4081 cggctatcaa agcctttgcc caaaagggtg gccatatttt gggtgtgtgc aacggcttcc
     4141 agattttaac ggaaacaggg cttctgcccg gtgctctgtt gcgcaacgcc aacctgcgct
     4201 ttctctctca ggattgctac ctgaaggtgg aagcagagaa cacacctttc actcacaagt
     4261 ggaagaaggg tgatgttttc cgcacccctc tggcccatgg tgatggcaat tacacggcag
     4321 atatcgccac gctggaacgg cttgaaggtg aaggtcttgt ggctttccac tatgccgatg
     4381 ccaaaggcaa tgtgcaggct gatgatgcca tcagcaaccc caatggcagc atcaacagca
     4441 ttgcgggtat tctgagcgaa aacaaacgca tttgcggcat gatgcctcat ccagaagatc
     4501 tggttgatcc tctcatgggt ggggaagatg gtctgccatt gttccaaggt ctggtggagg
     4561 ctgtagtccg gtgaataagc ctgtaaccgt tgatgaatcc cttgcccgtg aattcgggct
     4621 gacatctgag gaatacggtc gtgtgctgtc cattatgggc cgcaccccca gcctgacaga
     4681 gctgggcgtg ttctctgtga tgtggtcgga acactgctcc tacaaatctt cccgcaagtg
     4741 gctgcgcacg ctgccaacca aggcgccgtg ggttattcat ggccccggtg aaaatgctgg
     4801 tgtggtggat attgggcagg gctatgccgc catcttcaag atggaaagcc ataaccaccc
     4861 atcctttatc gaaccctatc agggtgcggc cacgggtgtg gggggcattc tgcgcgatgt
     4921 gttcaccatg ggcgcacgcc ccattgccaa cttgaacgcc ctgcgttttg gcagccccga
     4981 aaaccccgtt acccgccgcg tgattgatgg cgtggtgcgt ggtattggcg gctatggcaa
     5041 ctgtgttggc gtgcccaccg taggcgggga aatcaacttc cacccggcct atgatggcaa
     5101 cccgctggta aacgccatga cggttggttt ggcccgtaag gaccgtatct tcctttcggc
     5161 tgcggcaggt gttggcaacc cggttgttta cgtgggctcc cgcacagggc gtgatggcat
     5221 tcatggtgcc accatgtctt ccgccgagtt tgatgaagat gccctttcca agcgccccac
     5281 ggtacaggtg ggtgacccct ttattgaaaa gcttctgatt gaagcctgtc tggaactgat
     5341 ggcaacagat gccattatcg ccattcagga catgggcgct gcgggtctga catcttcttc
     5401 cgtggaaatg gcaggcaagg gcggtgtggg cattgagctt gatctggatg ccgtgcccca
     5461 gcgtgagccg aacatgacgg cttatgaaat gatgctgtct gaaagccagg aacgcatgct
     5521 gatggtgctg cgccctgacc ggacagacgt ggcccgcaag atctttgaaa aatgggaact
     5581 ggattttgcc attattgggc atctgacaga taccggccac atcgtcgtca agcacaaagg
     5641 acagttggaa gccgatattc cgctggaccc attggcagaa caggccccta tttacgatcg
     5701 gcccgcaaca ccacccaagg cgccggctcc gctagaaggc gtgcatgcac cgctttcttt
     5761 ggatgccacg ttactgaagc tggttggttc gccagatctg gcctcccgcg catgggtatg
     5821 gaaccagtat gacagcactg taggggggca gacagtccgc cgtccgggtg ctgctgatgc
     5881 tgccattgtg aaggtggaag atacagacat tggtctggcc atcaccacag attgcacccc
     5941 gcgttactgt caggcagacc ccaaaacagg tggcgcacaa gctgttgcag aagcatggcg
     6001 taacctaaca gctactggcg cacgcccgct ggccgtaacg gataacctga acttcgccaa
     6061 cccggaaaaa cccgaaatca tgggccagtt tgtggctgcg gttgaaggta tgggggaagc
     6121 ctgtacagcg ctggatttcc cggtcgtgag cggcaacgtg tctctttata atgaaacacg
     6181 aaacccagat ggctccacca ccgccattct gcccacaccc gccattggcg ccttgggcgt
     6241 tctggatgat gtgaacaagg ctgttggcct tggcatgcag ccagaatctg acgtggtgct
     6301 gattggtgaa accaagggtg agctcggcca gaccatctgg ctgcgtgaaa ttctgggtca
     6361 ggaagctggt gcaccgccgc cggtcgatct ggttgccgaa aaacgcaatg gtgactttgt
     6421 acgcagcgcc attctggatg gcaccattac cgcatgccat gacattgctg acggtggcat
     6481 tatggttgcc gccgcagaaa tggtgatggc aagtggcatt ggatgtgagc tggaaagccc
     6541 agattccggt atccggccag aagccttctg gtttggtgaa gatcaggccc gctatctggc
     6601 ttgtgtagca aacggcaaag ccctgtgcga acgcgctcag gcagctggtg tgcctgcacg
     6661 cattattgcc cgctcaggcg gaaatgattt gattctgccc agcggcgtca ccatatccgc
     6721 agcacgccta aaaacggccc atgaagcatt tttcccgatg ctgatgggca actaactcga
     6781 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     6841 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     6901 gaggggtttt ttgctgaaag gaggaactat atccggat
//
