LOCUS       sequence-132622-        5975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a membrane bound eGFP, under the UbC promoter. This 
            promoter expresses transgenes in neurons at higher levels than 
            plasmids with the CMV or beta-actin-CMV promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5975)
  TITLE     pAAV-UbC-eGFP-F
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-UbC-eGFP-F
FEATURES             Location/Qualifiers
     source          1..5975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        150..549
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1119..1138
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1835..1854
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1883..1885,1886..1888,1889..2599)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2189..2208)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2722..3198
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2921..2940)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3238..3378
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3238..3367
                     /label=AAV2 ITR
     rep_origin      3453..3908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3540..3559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3750..3771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3925..3944)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4044..4066
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4104..4122)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4190..4294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4295..4363,4364..5155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4513..4532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5326..5914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5815..5834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc
      181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     1201 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     1261 gtttttggct tttttgttag acgaagctaa ggcgcgcctg agaacttcag ggtgagtttg
     1321 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     1381 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tgcatggacc
     1441 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1501 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatcta
     1561 gacaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     1621 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     1681 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     1741 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     1801 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1861 catcaagctt ggatccaagc ttatggtgtc caagggcgag gaactgttca ccggcgtggt
     1921 gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg tgtccggcga
     1981 gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2041 gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc agtgcttcag
     2101 cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     2161 cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca gggccgaagt
     2221 gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg acttcaaaga
     2281 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     2341 catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc acaacatcga
     2401 ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gagatggccc
     2461 cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca aggaccccaa
     2521 cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca tcaccctggg
     2581 catggacgag ctgtacaaga gcggcctgcg gagcaagctg aacccccctg atgagtctgg
     2641 ccctggctgc atgagctgca aatgcgtgct gagctaatct agactcgagt ctagagttaa
     2701 cggccatatt ggccaatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     2761 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     2821 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     2881 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     2941 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3001 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3061 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3121 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3181 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3241 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3301 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3361 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3421 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3661 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3781 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     3901 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     3961 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4021 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4081 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4141 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4261 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4321 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4381 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4441 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4501 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4561 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4621 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4681 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4741 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4801 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4861 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4921 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4981 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5041 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5101 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5461 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5941 gttcctggcc ttttgctggc cttttgctca catgt
//
