LOCUS       sequence-13263-e        7225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-CAT SMR5453
FEATURES             Location/Qualifiers
     source          1..7225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2103..2762)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2677..2696
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2763..2865)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5246..5263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5417..6005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5497..5516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6176..6967,6968..7036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6799..6818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7037..7141)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7209..7225,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctggcgc gcctaggcca tatgggcccg ggcgagcata ttgatccgct gcaaactgaa
       61 attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg
      121 agctgccagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc
      181 tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc aaatggcgga agtaccaacc
      241 tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt
      301 acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctctat
      361 atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt gattaacttc
      421 aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt
      481 gtcggatgcg atgcttgcgc atcttatccg acctacgaat cgcatcgaat ctgtaggcca
      541 gataaggcat tttcgcagca tccggcactt attgtcggat gcgatgcttg cgcatcttat
      601 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
      661 atagttaatt gctcttactt cttcgcctct gcaaccactt tactacccac gccgcggtta
      721 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta
      781 tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac
      841 ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
      901 gtattggcat caacatccag cgagcggccc agttgcgcca caccatcacc gaaagcattg
      961 aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac
     1021 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
     1081 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
     1141 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
     1201 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc atcagtacgc
     1261 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
     1321 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagagca gagaacaccg
     1381 atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc
     1441 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gccaccatca
     1501 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
     1561 cccggagcgg cagggacata taccgttccc tgatactcac caggcatcac ggcaatatac
     1621 tggcgcttgt tggtacgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
     1681 gttacacctt gagtgcccgc cgggccgaca acaaagtcag gttgcgcagg cagggtaatc
     1741 ggggaaggat tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg
     1801 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcggtttga
     1861 tcagaaggac gttgatcggg cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc
     1921 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
     1981 agaaaaatga atccgttgaa ggcatgcctg caggtccgaa tttctgccat tcatccgctt
     2041 attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa
     2101 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     2161 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     2221 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2281 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     2341 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     2401 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     2461 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     2521 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg
     2581 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2641 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2701 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2761 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2821 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     2881 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     2941 aagtgatctt ccgtcacagg tatttattcg gactctagag ctcgaggtag atctgaaagc
     3001 ttgaatcccg gtgcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
     3061 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcagtaatcg
     3121 aggcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggcacctt
     3181 catcgacatc aatacgttct atatccagcg cgtgaacggt aaaaatgtag cgatgagttt
     3241 cgcctttcgg cggtgctgcg ccatcgtacc cggttttacc aaagtcggta cgcgtctgca
     3301 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
     3361 cagcgggtaa gttaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
     3421 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
     3481 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaatttat
     3541 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     3601 gtgtagccag aaaccctcac gcggacttct cgttattggc aaaaaaatgt ttcatcctgt
     3661 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     3721 ggcatcaggt aaatcagttt gccaaaaggc cggatccaga caccctgttc gacaaagaat
     3781 ttttgcagcg ccgccatatt caccggatga gtggtttcga ccacgccaat ggcccccagt
     3841 acgcgcacat cggcaaccat ttcggcatca cgggcggggg caagttgctc gcgcagctgt
     3901 acttcaatat ccgccacctg ttgctgccag tcgccagatt cgagaatcgc caggctggcg
     3961 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcaaccg
     4021 gcttcaccgt tactgatggt ttctgcaacc tcgcgcgtgg tgagtgtggc ggaaagggtc
     4081 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatgt
     4141 tcacaggcaa acagtttccc ggtacgacca aatccagtgg cgatctcgtc ggcaatcagc
     4201 aagatacctt cgcgatcgca tattttgcgg attcgtttta accattccgg atggtacatg
     4261 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgccgcgat ttcatgacga
     4321 tgcgccgcca tcaggcgggc aaagcccacc atatcgcgct catcccattc gccatccatg
     4381 cggctttgcg gggcgggagc aaacaggttt tctggcaggt agcctttcca cagactgtgc
     4441 attgagttat ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     4501 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     4561 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     4621 tgcggcgtca tcgccaccag tttgcggcac agctcaatgg ctggcgcatg ggtgataccg
     4681 ccaaacatca catgcgacat ggcatcaatt tgcgacttca tcgccgcatt aagctgcggg
     4741 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     4801 tcagacaaaa tcagctcgca accttcggcg ctcaccaccg gataaaccgg cagaggggag
     4861 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     4921 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagtctaca ccgaattaac
     4981 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     5041 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     5101 tcgccagcat ttcgatcctc gtcaggtgca ggtcagcacg ttgctgcata tggcgcgcct
     5161 aggccaattg ggcccgggca tatgggtgtg aaataccgca cagatgcgta aggagaaaat
     5221 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6481 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6781 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7141 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7201 cctataaaaa taggcgtatc acgag
//
