LOCUS       sequence-132631-        6861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 OrfY; formylglycinamidine 
            ribonucleotide synthetase glutaminase subunit; large subunit with 
            C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK624
FEATURES             Location/Qualifiers
     source          1..6861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2472..2491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2632..2654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3348..3367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3348..3366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3398..3420
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6705..6722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6775..6793)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6789..6836
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
     3361 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
     3421 tatacatatg aagcgcaccc ttcttatagc gctggcattg gcatgcgcgg caccgggctt
     3481 ggcccatgcc cagcgcattg cgcccatgaa ggcaggtaac tttgcccgcc tttgtacgcg
     3541 cgccactagt gctggcattt gcgatgccta tattggcggc ctgacggatg gggtctctct
     3601 ttccaaaatc aatgatcgga acgaggggga tccttctgcg cctgctggtt tttgtgttcc
     3661 cgctacagaa accatgcccg ttatgcgctc caaggtactt acctggctga aagcgcattc
     3721 tgaaacactg gacaagccgg ttggtgaaag tgtttttgcc gccctgcacg cctcttatcc
     3781 gtgcagtgtg aaaaaatgaa agcagcaatt atcgtattcc ccggtaccaa ccgggagcgc
     3841 gatatggcca ttgccctcaa gcgcgttaca gggcaggccc cgcgcatgat ctggcaccat
     3901 gaaacagatc tgggagatac cgatctagtc gtgctgccag gtggtttcag ctatggcgat
     3961 tatctgcgct gtggtgccat ggcagcgcat tctcccatca tggcggctat caaagccttt
     4021 gcccaaaagg gtggccatat tttgggtgtg tgcaacggct tccagatttt aacggaaaca
     4081 gggcttctgc ccggtgctct gttgcgcaac gccaacctgc gctttctctc tcaggattgc
     4141 tacctgaagg tggaagcaga gaacacacct ttcactcaca agtggaagaa gggtgatgtt
     4201 ttccgcaccc ctctggccca tggtgatggc aattacacgg cagatatcgc cacgctggaa
     4261 cggcttgaag gtgaaggtct tgtggctttc cactatgccg atgccaaagg caatgtgcag
     4321 gctgatgatg ccatcagcaa ccccaatggc agcatcaaca gcattgcggg tattctgagc
     4381 gaaaacaaac gcatttgcgg catgatgcct catccagaag atctggttga tcctctcatg
     4441 ggtggggaag atggtctgcc attgttccaa ggtctggtgg aggctgtagt ccggtgaata
     4501 agcctgtaac cgttgatgaa tcccttgccc gtgaattcgg gctgacatct gaggaatacg
     4561 gtcgtgtgct gtccattatg ggccgcaccc ccagcctgac agagctgggc gtgttctctg
     4621 tgatgtggtc ggaacactgc tcctacaaat cttcccgcaa gtggctgcgc acgctgccaa
     4681 ccaaggcgcc gtgggttatt catggccccg gtgaaaatgc tggtgtggtg gatattgggc
     4741 agggctatgc cgccatcttc aagatggaaa gccataacca cccatccttt atcgaaccct
     4801 atcagggtgc ggccacgggt gtggggggca ttctgcgcga tgtgttcacc atgggcgcac
     4861 gccccattgc caacttgaac gccctgcgtt ttggcagccc cgaaaacccc gttacccgcc
     4921 gcgtgattga tggcgtggtg cgtggtattg gcggctatgg caactgtgtt ggcgtgccca
     4981 ccgtaggcgg ggaaatcaac ttccacccgg cctatgatgg caacccgctg gtaaacgcca
     5041 tgacggttgg tttggcccgt aaggaccgta tcttcctttc ggctgcggca ggtgttggca
     5101 acccggttgt ttacgtgggc tcccgcacag ggcgtgatgg cattcatggt gccaccatgt
     5161 cttccgccga gtttgatgaa gatgcccttt ccaagcgccc cacggtacag gtgggtgacc
     5221 cctttattga aaagcttctg attgaagcct gtctggaact gatggcaaca gatgccatta
     5281 tcgccattca ggacatgggc gctgcgggtc tgacatcttc ttccgtggaa atggcaggca
     5341 agggcggtgt gggcattgag cttgatctgg atgccgtgcc ccagcgtgag ccgaacatga
     5401 cggcttatga aatgatgctg tctgaaagcc aggaacgcat gctgatggtg ctgcgccctg
     5461 accggacaga cgtggcccgc aagatctttg aaaaatggga actggatttt gccattattg
     5521 ggcatctgac agataccggc cacatcgtcg tcaagcacaa aggacagttg gaagccgata
     5581 ttccgctgga cccattggca gaacaggccc ctatttacga tcggcccgca acaccaccca
     5641 aggcgccggc tccgctagaa ggcgtgcatg caccgctttc tttggatgcc acgttactga
     5701 agctggttgg ttcgccagat ctggcctccc gcgcatgggt atggaaccag tatgacagca
     5761 ctgtaggggg gcagacagtc cgccgtccgg gtgctgctga tgctgccatt gtgaaggtgg
     5821 aagatacaga cattggtctg gccatcacca cagattgcac cccgcgttac tgtcaggcag
     5881 accccaaaac aggtggcgca caagctgttg cagaagcatg gcgtaaccta acagctactg
     5941 gcgcacgccc gctggccgta acggataacc tgaacttcgc caacccggaa aaacccgaaa
     6001 tcatgggcca gtttgtggct gcggttgaag gtatggggga agcctgtaca gcgctggatt
     6061 tcccggtcgt gagcggcaac gtgtctcttt ataatgaaac acgaaaccca gatggctcca
     6121 ccaccgccat tctgcccaca cccgccattg gcgccttggg cgttctggat gatgtgaaca
     6181 aggctgttgg ccttggcatg cagccagaat ctgacgtggt gctgattggt gaaaccaagg
     6241 gtgagctcgg ccagaccatc tggctgcgtg aaattctggg tcaggaagct ggtgcaccgc
     6301 cgccggtcga tctggttgcc gaaaaacgca atggtgactt tgtacgcagc gccattctgg
     6361 atggcaccat taccgcatgc catgacattg ctgacggtgg cattatggtt gccgccgcag
     6421 aaatggtgat ggcaagtggc attggatgtg agctggaaag cccagattcc ggtatccggc
     6481 cagaagcctt ctggtttggt gaagatcagg cccgctatct ggcttgtgta gcaaacggca
     6541 aagccctgtg cgaacgcgct caggcagctg gtgtgcctgc acgcattatt gcccgctcag
     6601 gcggaaatga tttgattctg cccagcggcg tcaccatatc cgcagcacgc ctaaaaacgg
     6661 cccatgaagc atttttcccg atgctgatgg gcaactcact cgagcaccac caccaccacc
     6721 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     6781 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6841 aaggaggaac tatatccgga t
//
