LOCUS       sequence-132637-       11022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MSTN TALEN with 2A-GFP tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Xu L, Zhao P, Mariano A, Han R
  TITLE     Targeted Myostatin Gene Editing in Multiple Mammalian Species 
            Directed by a Single Pair of TALE Nucleases.
  JOURNAL   Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 
            10.1038/mtna.2013.39.
  PUBMED    23900226
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2013-07-30"; volume: "2:e112. doi"; pages: "
            10.1038/mtna.2013.39"
COMMENT     Sequence Label: pEGFP-2A-TAL6-MSTN
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1330..1389
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             1399..1464
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1471..1491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3808..4395
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNPTQDRILEMKV
                     MEFFMKVYGYRGEHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             4411..4464
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4471..4536
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4543..4563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7084..7671
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNPTQDRILEMKV
                     MEFFMKVYGYRGEHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNRKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     polyA_signal    7810..7931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7847..7866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7901..7920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7938..8393)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8075..8096)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8287..8306
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8420..8524
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8526..8883
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8526..8717
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8549..8569)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8734..8869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8918..9712
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8918..9712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8972..8991)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9582..9601
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9900..9919)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9944..9991
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10320..10908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10809..10828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagc aatgtactaa ctacgctttg ttgaaactcg ctggcgatgt tgaaagtaac
     1381 cccggtcctg aattcatgga ctacaaagac catgacggtg attataaaga tcatgacatc
     1441 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
     1501 ggggtaccta tggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc
     1561 aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt
     1621 acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc
     1681 acgtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc
     1741 ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg
     1801 agaggtccgc cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc
     1861 gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc tcccctgaac
     1921 ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa
     1981 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     2041 gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     2101 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     2161 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     2221 catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg
     2281 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     2341 caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg
     2401 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     2461 agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     2521 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
     2581 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     2641 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
     2701 cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca agtggtggct
     2761 atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     2821 ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc
     2881 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     2941 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
     3001 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     3061 gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     3121 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     3181 attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     3241 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
     3301 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     3361 caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg
     3421 ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc
     3481 agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     3541 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag
     3601 caagcgctcg aaagcattgt ggcccagctg agccggcctg atccggcgtt ggccgcgttg
     3661 accaacgacc acctcgtcgc cttggcctgc ctcggcggac gtcctgccat ggatgcagtg
     3721 aaaaagggat tgccgcacgc gccggaattg atcagaagag tcaatcgccg tattggcgaa
     3781 cgcacgtccc atcgcgttgc cggatcccag ctggtcaaaa gtgaactgga ggagaagaaa
     3841 tctgaacttc gtcataaatt gaaatatgtg cctcatgaat atattgaatt aattgaaatt
     3901 gccagaaatc ccactcagga taggattctt gaaatgaagg taatggaatt ttttatgaaa
     3961 gtttatggat atagaggtga acatttgggt ggatcaagga aaccggacgg agcaatttat
     4021 actgtcggat ctcctattga ttacggtgtg atcgtggata ctaaagctta tagcggaggt
     4081 tataatctgc caattggcca agcagatgaa atggaacgat atgtcgaaga aaatcaaaca
     4141 cgagacaaac atctcaaccc taatgaatgg tggaaagtct atccatcttc tgtaacggaa
     4201 tttaagtttt tatttgtgag tggtcacttt aaaggaaact acaaagctca gcttacacga
     4261 ttaaatcata tcactaattg taatggagct gttcttagtg tagaagagct tttaattggt
     4321 ggagaaatga ttaaagccgg cacattaacc ttagaggaag tgagaaggaa atttaataac
     4381 ggcgagataa actttagatc tggcggcgga gagggcagag gaagtcttct aacatgcggt
     4441 gacgtggagg agaatcccgg ccctaggatg gactacaaag accatgacgg tgattataaa
     4501 gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag
     4561 gtgggcattc acggggtacc tatggtggat ctacgcacgc tcggctacag tcagcagcag
     4621 caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg
     4681 ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg
     4741 accgtcgctg tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac
     4801 atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat
     4861 gcgggggagt tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca
     4921 aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcat cgcgcaatgc actgacgggt
     4981 gctcccctga acctgacccc ggaccaagtg gtggctatcg ccagccacga tggcggcaag
     5041 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact
     5101 ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg
     5161 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
     5221 atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     5281 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc
     5341 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     5401 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
     5461 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     5521 gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     5581 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     5641 ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     5701 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
     5761 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac
     5821 caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     5881 ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc
     5941 agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     6001 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag
     6061 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     6121 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
     6181 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
     6241 atcgccagca acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     6301 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc
     6361 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     6421 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
     6481 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg
     6541 gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     6601 ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac
     6661 gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     6721 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
     6781 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     6841 caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaag cattgtggcc
     6901 cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca acgaccacct cgtcgccttg
     6961 gcctgcctcg gcggacgtcc tgccatggat gcagtgaaaa agggattgcc gcacgcgccg
     7021 gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca cgtcccatcg cgttgccgga
     7081 tcccagctgg tcaaaagtga actggaggag aagaaatctg aacttcgtca taaattgaaa
     7141 tatgtgcctc atgaatatat tgaattaatt gaaattgcca gaaatcccac tcaggatagg
     7201 attcttgaaa tgaaggtaat ggaatttttt atgaaagttt atggatatag aggtgaacat
     7261 ttgggtggat caaggaaacc ggacggagca atttatactg tcggatctcc tattgattac
     7321 ggtgtgatcg tggatactaa agcttatagc ggaggttata atctgccaat tggccaagca
     7381 gatgaaatgc aacgatatgt caaggaaaat caaacacgaa acaaacatat caaccctaat
     7441 gaatggtgga aagtctatcc atcttctgta acggaattta agtttttatt tgtgagtggt
     7501 cactttaaag gaaactacaa agctcagctt acacgattaa atcgtaagac taattgtaat
     7561 ggagctgttc ttagtgtaga agagctttta attggtggag aaatgattaa agccggcaca
     7621 ttaaccttag aggaagtgag aaggaaattt aataacggcg agataaactt ttaactcgag
     7681 tgatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     7741 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     7801 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7861 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7921 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     7981 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8041 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8101 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8161 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8221 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8281 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8341 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     8401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8521 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8701 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8761 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8821 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8881 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     8941 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     9001 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     9061 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     9121 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     9181 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     9241 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     9301 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     9361 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     9421 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     9481 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     9541 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     9601 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     9661 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     9721 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     9781 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9841 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     9901 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     9961 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    10021 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    10081 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    10141 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
    10201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    10501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10981 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
