LOCUS       sequence-132639-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary destination vector for plant transformation. Kanamycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: pCAM-kan-R4R3
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..232)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(241..257)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(242..258)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(282..301)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(283..301)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(311..328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(311..327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(320..342)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcaacttttc tatacaaagt tgaagcttat
      241 cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
      301 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      361 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      421 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
      481 taatgttatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac
      541 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga
      601 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
      661 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
//
