LOCUS       sequence-13264-e        6583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-ProK Express SMR5454
FEATURES             Location/Qualifiers
     source          1..6583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2029..2313
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2236..2255
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(4604..4621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4775..5363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4855..4874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5534..6325,6326..6394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6157..6176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6395..6499)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(6567..6583,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctggcgc gcctaggcca tatgggcccg ggcgagcata ttgatccgct gcaaactgaa
       61 attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg
      121 agctgccagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc
      181 tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc aaatggcgga agtaccaacc
      241 tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt
      301 acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctctat
      361 atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt gattaacttc
      421 aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt
      481 gtcggatgcg atgcttgcgc atcttatccg acctacgaat cgcatcgaat ctgtaggcca
      541 gataaggcat tttcgcagca tccggcactt attgtcggat gcgatgcttg cgcatcttat
      601 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
      661 atagttaatt gctcttactt cttcgcctct gcaaccactt tactacccac gccgcggtta
      721 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta
      781 tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac
      841 ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
      901 gtattggcat caacatccag cgagcggccc agttgcgcca caccatcacc gaaagcattg
      961 aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac
     1021 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
     1081 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
     1141 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
     1201 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc atcagtacgc
     1261 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
     1321 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagagca gagaacaccg
     1381 atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc
     1441 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gccaccatca
     1501 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
     1561 cccggagcgg cagggacata taccgttccc tgatactcac caggcatcac ggcaatatac
     1621 tggcgcttgt tggtacgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
     1681 gttacacctt gagtgcccgc cgggccgaca acaaagtcag gttgcgcagg cagggtaatc
     1741 ggggaaggat tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg
     1801 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcggtttga
     1861 tcagaaggac gttgatcggg cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc
     1921 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
     1981 agaaaaatga atccgttgaa ggcatgctct agctcccgcc attcagagaa gaaaccaatt
     2041 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     2101 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     2161 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     2221 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     2281 gctttttatc gcaactctct actgtttctc catacccgtt ttctagagct cgaggtagat
     2341 ctgaaagctt gaatcgatgt acgtaccggt gcgaaacggg aaggtaaaaa gacaaaaagt
     2401 tgtttttaat acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac
     2461 taaacatcgc agtaatcgag gcgcttgcca gagagtggaa atgaacgtta aacccgacca
     2521 tcgcgccgct ggcaccttca tcgacatcaa tacgttctat atccagcgcg tgaacggtaa
     2581 aaatgtagcg atgagtttcg cctttcggcg gtgctgcgcc atcgtacccg gttttaccaa
     2641 agtcggtacg cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt
     2701 gcggtaatac gcgggtatca gcgggtaagt taacaactac ccagtgccac cagccggagc
     2761 cggttggcgc atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat
     2821 catcccacgc cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat
     2881 gacgatgcgg caatttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc
     2941 ctttcttgtt tgcagaaagt gtagccagaa accctcacgc ggacttctcg ttattggcaa
     3001 aaaaatgttt catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc
     3061 gggagaataa tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccagaca
     3121 ccctgttcga caaagaattt ttgcagcgcc gccatattca ccggatgagt ggtttcgacc
     3181 acgccaatgg cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggcgggggca
     3241 agttgctcgc gcagctgtac ttcaatatcc gccacctgtt gctgccagtc gccagattcg
     3301 agaatcgcca ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc
     3361 ccatgcataa agcaaccggc ttcaccgtta ctgatggttt ctgcaacctc gcgcgtggtg
     3421 agtgtggcgg aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc
     3481 ggcgcgattt ctgcatgttc acaggcaaac agtttcccgg tacgaccaaa tccagtggcg
     3541 atctcgtcgg caatcagcaa gataccttcg cgatcgcata ttttgcggat tcgttttaac
     3601 cattccggat ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc
     3661 gccgcgattt catgacgatg cgccgccatc aggcgggcaa agcccaccat atcgcgctca
     3721 tcccattcgc catccatgcg gctttgcggg gcgggagcaa acaggttttc tggcaggtag
     3781 cctttccaca gactgtgcat tgagttatcc ggatcgcaca ccgacatcgc gccaaaggta
     3841 tcgccatgat aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc
     3901 cagtactgca acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga
     3961 aaaacgcact ccagcggttg cggcgtcatc gccaccagtt tgcggcacag ctcaatggct
     4021 ggcgcatggg tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgacttcatc
     4081 gccgcattaa gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg
     4141 tcaaccaggc gtctgccgtc agacaaaatc agctcgcaac cttcggcgct caccaccgga
     4201 taaaccggca gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag
     4261 gcaagatcgt ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca
     4321 agtctacacc gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc
     4381 acgctggaca ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt
     4441 tgaagcgcag caggtgcatc gccagcattt cgatcctcgt caggtgcagg tcagcacgtt
     4501 gctgcatatg gcgcgcctag gccaattggg cccgggcata tgggtgtgaa ataccgcaca
     4561 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     4621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5821 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     5881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6121 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     6181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6541 ttattatcat gacattaacc tataaaaata ggcgtatcac gag
//
