LOCUS       sequence-132640-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of two gRNAs from Drosophila U6:1 and U6:3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD4-U6:1_U6:3tandemgRNAs
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    287..672
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
       61 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
      121 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggct
      181 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
      241 agccgaattg atccactaga aggcctaatt cggtacacta gttggccacg taataagtgt
      301 gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata
      361 ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat
      421 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg
      481 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta
      541 ataaaataca ttgcataccc tcttttaata aagaatattg catacgttga cgaaacaaat
      601 tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc cataccattt
      661 agccgatcaa ttgagatctg aattcatttt caacgtcctc gatagtatag tggttagtat
      721 ccccgcctgt cacgcgggag accggggttc aattccccgt cggggagaat ctgtgattct
      781 tttttttttt cttttacttt gttatataaa caatttttgt tttaattgaa tctaatttgc
      841 cattgctttt aggaatctca ggcatccagc aagcgtttgt ccgccgaatc gcccatcagt
      901 gaagaagatc ctgtggcggc tacgaaaatc tccccggcca tgtcggcctc cacctccagc
      961 gaaaaaccca tcagcgagct ggccacctct gtgctgaccc accgctttcc agactccacc
     1021 tcctcacccg gcgaacatgg ccttggacga atgcagttgt cgatccgcta cagcgcccag
     1081 cgtcaaaaac tagacgtgac catacacaaa atccagaaga taccacttcg cgatcc
//
