LOCUS       sequence-132642-        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of two gRNAs from Drosophila U6:1 and U6:3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD4-U6:1_U6:3tandemgRNAs
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(300..375)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(394..814)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(815..890)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggagctcctg caggttgttg gttggcacac cacaaatata ctgttgccga gcacaattgt
       61 ctagaatgca tacgcattaa gcgaacatta aaaagatgtg aaaacataac tattatgtct
      121 aaataaacac acgtcagatg tatgtacgtc aacggaaaac cattgtctat atattacaat
      181 tactaaatac ataccaaatt gaatacatat tgatgaaaaa taataaatac tggcgaaagc
      241 aaaaaaacga aacattttta ttttattgaa caactctcag gctccaggta ggcaaaaaag
      301 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
      361 ttctagctct aaaacaggtc ttctcgaaga ccccgacgtt aaattgaaaa taggtctata
      421 tatacgaact gagtctggaa aaagaagttg agaattataa aaagtagtga gcactggcgc
      481 cctctctgct tggcgagcta accttttcgc ctcttggctg agtaggtggc gtttcattct
      541 actctgtaaa attaatgtag aattgaaaca ctatggtcaa aaaatactta ggggcataag
      601 ttataaaacg tatgaaatga atttttatca acctgggcta ttcaaaaatt ttcgaattat
      661 tttatgtatt ttttttaatc gtttttcatt ataggttaaa atacacttta aaaggaattc
      721 tttcctgtaa aataaatata aataaatatg ctttattgac agaaaatttg atgtttttgt
      781 atttgagtag gagcaatcac aggtgagcaa aaaagcaccg actcggtgcc actttttcaa
      841 gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac aggtcttctc
      901 gaagaccccg aagttcaccc ggatatcttt cctatatata ctgtactctt gcagaatatg
      961 tacttgggaa tcataaggat gcgaaggacg tacgcgctgt gtctgtaact aaggggttgc
     1021 tttcgggtac ctaccatgcc aatgatggga ctaccgccgg ataggaatcc cttttgggtg
     1081 cacaccgtca cctccagttc cgtctgcctg agttcggcaa tggaaa
//
