LOCUS       sequence-132647-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - chloramphenicol resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-cat
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..52
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(137..156)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcctgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
       61 gaactaaacc atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      121 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      181 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      241 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      301 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      361 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      421 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      481 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      541 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      601 tacgcaaggc
//
