LOCUS       sequence-132649-         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - chloramphenicol resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-cat
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(59..92)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(114..130)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(115..131)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        217..321
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatttcgacg acctgcagcc aagctagctt ggctggacgt aaactcctct tcagacctga
       61 agttcctata ctttctagag aataggaact tcggaattcg atatcaagct tatcgatacc
      121 gtcgacctcg agggggggcc cggtaccttc accgtttgga ccttggggcc gtaattaccc
      181 tgttatccct aaggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      241 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      301 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      361 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      421 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      481 atccttgaga gttttcgccc c
//
