LOCUS       sequence-132650-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C9orf114 with FLAG-tag epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-C9orf114-flag
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    416..879
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(576..595)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     805..822
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcgacttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc
       61 ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc
      121 tgtcggcacc gtccgcaggg gctcaagatg cccctgnttc tcatttccga tcgcgacgat
      181 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
      241 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
      301 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
      361 tctttcaatg agggtggatt cttcttgaga caaaggaacc atggttgtgg ccatattatc
      421 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
      481 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
      541 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
      601 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
      661 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
      721 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
      781 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
      841 tgctcgtcaa gaagacaggg ccaggtttcc ngggccctca cattgccaaa agacggcaat
      901 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gct
//
