LOCUS       sequence-132658-        5215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acetobacter aceti 1023 mutS, partial gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5215)
  TITLE     Acetobacter aceti 1023 genetic tools
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK637
FEATURES             Location/Qualifiers
     source          1..5215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(856..1671)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(856..1671)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1579..1598
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1663..1682
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(1817..2608,2609..2677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2440..2459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2678..2782)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2809..3264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2896..2915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3106..3127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3390..3412
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3404..3421
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3405..3421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3435..3453
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3435..3452
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4958..4977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4959..4977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4996..5012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4996..5012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5009..5031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5020..5036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5044..5050,5051..5068,5069..5074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5089..5110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      901 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      961 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1021 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1081 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1141 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1201 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     1261 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1321 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     1381 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     1441 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     1501 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     1561 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     1621 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct
     1681 tgtattactg tttatgtaag cagacagttt tattgttcat gagattatca aaaaggatct
     1741 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1801 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1861 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1921 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1981 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2041 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2101 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2161 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2221 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2281 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2341 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2401 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2461 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2521 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2581 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2641 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2701 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2761 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     2821 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2881 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2941 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3001 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3061 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3121 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3181 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3241 acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
     3301 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     3361 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     3421 tgaattgtgc ggccatttag gtgacactat agaatacagc ggccgcgagc tcgggccccc
     3481 acacgtgtgg tctagagcta gcctaggctc gagaagcttg tcgacgaatt cagatccatc
     3541 gattggatcc tgcagaaatt ctggcgcccg ccattcttga tctggcggat tacgaggccc
     3601 ggcgtgcgcc agaaacggtg gcataaggcg cggatacagc tcgccagcgt ttggccaatg
     3661 cctttgatgt ggccagtata gaagcctttg gcacttttac ggatgaggaa agcgtggccg
     3721 ctgctatggc agtggaatat gtgcagcgca gtcaggctgg caagttgcca cgtttggcgc
     3781 atccggttcc gcagaatgaa agcgggattc tggggataga tccggccaca cgtgccagtt
     3841 tggatatttt gcgtgcgcga gacggagggg aggaacatac gttattttcc gccgtaaacc
     3901 gtacggtcag tgccgcaggg gcgcgtatgc tggcggaatg gctggccgcc ccccttacac
     3961 atgcagggca gattgctgcc cggcaggatg gctggtggtg gctgaaagaa gattccgtgc
     4021 gccgagaggc attgcgcagc acacttaaaa aagcccctga tattgcacgc gcactcggtc
     4081 gcttatctct ggggcgcggg caaccgcgag atctggcagc catgcgtgat ggccttatgg
     4141 tggcgcggga agctgcacgc attcttacgg ggggcaacgc ctctttgccg ccagccatac
     4201 atcaggctgt tacacggttg ggcggagggc aggcgcttga agccgaactg gtgcgcgcgt
     4261 tggcggaaaa tcttccggcg cggttggatg atggtggtgt tattgcacaa ggctatgatg
     4321 ccaagctgga tgaataccgt ggattgcgtg atgattcgcg ccgtctgatc gcgggtttgc
     4381 aggccaaata tgctgcgcat tacgggttaa atagccttaa aattcgccat cacgcgcagc
     4441 ttggttatgt catggaagtg cctgtggctg ccagcgccaa actgaaggat aagccagaac
     4501 tgatcttgcg gcagggtacg gccagcaacg cgcgttttgc tacggaagaa ctctcaacgc
     4561 tggatgcccg cataacagaa gcagcagaga aggccgcgca acgggaaaaa gaattgttca
     4621 gtaggctggt tgcgcaggtt ctggcagagc gtagtatgcc ggttctggca caggctttgg
     4681 ctgtattgga tgttttacag tcctgtgctg atctggcggg gaatggcaca tggtgccgac
     4741 cagaagtgac ggaagattgt gcatttgcct tggaaggatg ccgccacccg gtggtggaag
     4801 ctgccctgcc agcaggcaca cgctttacgc ccaacacgtg tgatctggca ccgcaacagc
     4861 gcgttatgct gctaaccggg ccaaatatgg actagtttaa tcacgaattc tggatccgat
     4921 acgtaacgcg tctgcagcat gcgtggtacc gagctttccc tatagtgagt cgtattagag
     4981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgc
//
