LOCUS       sequence-132661-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - hygromycin resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-hph
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(57..90)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(112..128)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        215..319
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(538..557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttcgacgac ctgcagccaa gctagcttgg ctggacgtaa actcctcttc agacctgaag
       61 ttcctatact ttctagagaa taggaacttc ggaattcgat atcaagctta tcgataccgt
      121 cgacctcgag ggggggcccg gtaccttcac cgtttggacc ttggggccgt aattaccctg
      181 ttatccctaa ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      541 atgtggcgcg gtattat
//
