LOCUS       sequence-132664-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - trimethoprim resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-dhfr
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..68
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             87..323
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     protein_bind    357..390
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggcctttcga cctgcagcct gttgacaatt aatcatcggc atagtatatc ggcatagtat
       61 aatacgacaa ggtgaggaac taaaccatgg gtcaaagtag cgatgaagcc aacgctcccg
      121 ttgcagggca gtttgcgctt cccctgagtg ccacctttgg cttaggggat cgcgtacgca
      181 agaaatctgg tgccgcttgg cagggtcaag tcgtcggttg gtattgcaca aaactcactc
      241 ctgaaggcta tgcggtcgag tccgaatccc acccaggctc agtgcaaatt tatcctgtgg
      301 ctgcacttga acgtgtggcc taatgagggg atcaattctc tagagctcgc tgatcagaag
      361 ttcctattct ctagaaagta taggaacttc gatggcgcct catccctgaa gccaataggg
      421 ataacagggt aatgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
      481 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
      541 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
      601 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
      661 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
      721 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
      781 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
      841 tgatgacggt ga
//
