LOCUS       sequence-132668-        1443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-HA-Sestrin2
FEATURES             Location/Qualifiers
     source          1..1443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatcgtgg cggactccga gtgccgcgca gagctcaagg actacctgcg gttcgccccg
       61 ggcggcgtcg gcgactcggg ccccggagag gagcagaggg agagccgggc tcggcgaggc
      121 cctcgagggc ccagcgcctt catccccgtg gaggaggtcc ttcgggaggg ggctgagagc
      181 ctcgagcagc acctggggct ggaggcactg atgtcctctg ggcgagtaga caacctggca
      241 gtggtgatgg gcctgcaccc tgactacttt accagcttct ggcgcctgca ctacctgctg
      301 ctgcacacgg atggtccctt ggccagctcc tggcgccact acattgccat catggctgcc
      361 gcccgccatc agtgttctta cctggtaggc tcccacatgg ccgagtttct gcagactggt
      421 ggtgaccctg agtggctgct gggcctccac cgggcccccg agaagctgcg caaactcagc
      481 gagatcaaca agttgctggc gcatcggcca tggctcatca ccaaggaaca catccaggcc
      541 ttgctgaaga ccggcgagca cacttggtcc ctggccgagc tcattcaggc tctggtcctg
      601 ctcacccact gccactcgct ctcctccttc gtgtttggct gtggcatcct ccctgagggg
      661 gatgcagatg gcagccctgc cccccaggca cctacacccc ctagtgaaca gagcagcccc
      721 ccaagcaggg acccgttgaa caactctggg ggctttgagt ctgcccgcga cgtggaggcg
      781 ctgatggagc gcatgcagca gctgcaggag agcctgctgc gggatgaggg gacgtcccag
      841 gaggagatgg agagccgctt tgagctggag aagtcagaga gcctgctggt gaccccctca
      901 gctgacatcc tggagccctc tccacaccca gacatgctgt gctttgtgga agaccctact
      961 ttcggatatg aggacttcac tcggagaggg gctcaggcac cccctacctt ccgggcccag
     1021 gattatacct gggaagacca tggctactcg ctgatccagc ggctttaccc tgagggtggg
     1081 cagctgctgg atgagaagtt ccaggcagcc tatagcctca cctacaatac catcgccatg
     1141 cacagtggtg tggacacctc cgtgctccgc agggccatct ggaactatat ccactgcgtc
     1201 tttggcatca gatatgatga ctatgattat ggggaggtga accagctcct ggagcggaac
     1261 ctcaaggtct atatcaagac agtggcctgc tacccagaga agaccacccg aagaatgtac
     1321 aacctcttct ggaggcactt ccgccactca gagaaggtcc acgtgaactt gctgctcctg
     1381 gaggcgcgca tgcaagccgc tctgctgtac gccctccgtg ccatcacccg ctacatgacc
     1441 tga
//
