LOCUS       sequence-132670-        1656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin1 isoform1
FEATURES             Location/Qualifiers
     source          1..1656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccgaag gagaaaatga agtgcggtgg gatgggctgt gctcaaggga ctcaacaaca
       61 agggagactg ctctggaaaa tatcaggcag acaatcctga gaaaaactga atatctgagg
      121 agtgtgaagg agactcccca caggcctagt gacgggctgt caaacaccga gagctccgat
      181 ggactgaata agctgctggc tcatctgctg atgctgtcaa agaggtgccc tttcaaagat
      241 gtgcgcgaaa agagcgagtt tatcctgaaa tccattcagg agctgggcat caggattcca
      301 cgccccctgg gacagggacc aagcaggttt atccccgaaa aggagattct gcaagtggga
      361 tccgaggacg cccagatgca cgctctgttc gcagattctt ttgccgctct gggccgcctg
      421 gacaacatca ccctggtcat ggtcttccac ccacagtatc tggagtcttt tctgaaaacc
      481 cagcattacc tgctgcagat ggacggacct ctgccactgc actaccgcca ttatatcggc
      541 attatggcag ccgctcgaca ccagtgttcc tacctggtga acctgcacgt caatgacttc
      601 ctgcatgtgg gcggggatcc taagtggctg aacgggctgg aaaatgcacc acagaaactg
      661 cagaacctgg gagagctgaa taaggtgctg gcacaccgac catggctgat cacaaaagaa
      721 catattgagg ggctgctgaa ggctgaggaa cactcatgga gcctggccga actggtgcat
      781 gctgtggtcc tgctgaccca ctatcattcc ctggcatctt tcacatttgg ctgcgggatc
      841 tccccagaga ttcactgtga tggaggccat acctttagac ccccttccgt gtctaattac
      901 tgcatctgtg atattacaaa cggcaatcac tctgtggacg aaatgcccgt caacagtgcc
      961 gagaatgtga gtgtctcaga tagcttcttt gaagtggagg ctctgatgga aaaaatgcgc
     1021 cagctgcagg agtgccgaga cgaggaagag gcatctcagg aagagatggc cagtcggttc
     1081 gaaatcgaga agagagaaag catgttcgtg ttttctagtg acgatgaaga ggtgacaccc
     1141 gcacgagccg tctcacggca cttcgaggac actagctacg gatataagga tttttccaga
     1201 cacggcatgc atgtgcccac attcagggtc caggactact gttgggaaga tcatggctat
     1261 tccctggtga accgcctgta ccctgacgtg gggcagctga tcgatgagaa attccacatt
     1321 gcttacaacc tgacctataa tacaatggca atgcataagg acgtggatac ttctatgctg
     1381 cggagagcca tctggaacta tattcactgc atgtttggca tccggtacga cgattacgat
     1441 tatggggaaa ttaatcagct gctggacaga agtttcaagg tgtatatcaa aactgtggtc
     1501 tgtacccctg agaaggtcac caaacgaatg tacgactcat tctggcggca gtttaagcac
     1561 agcgaaaaag tgcatgtcaa tctgctgctg attgaggcac gaatgcaggc tgaactgctg
     1621 tatgctctga gggctattac tcggtatatg acttga
//
