LOCUS       sequence-132671-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(198..273)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(295..543)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(353..372)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(523..543)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(685..704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacgtcaatg gaaagtccct attggcgtta ctattgacgt caatgggcgg gggtcgttgg
       61 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gggtacctct agagccattt
      121 gtctgcagaa ttggcgcacg cgctaaaaac ggactagcct tattttaact tgctatttct
      181 agctctaaaa caaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      241 cttattttaa cttgctattt ctagctctaa aactggaaag ttgttccatt caacggtgtt
      301 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      361 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      421 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat
      481 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      541 ctcacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cag
//
