LOCUS       sequence-132673-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Fujita T, Kitaura F, Fujii H
  TITLE     A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression
            of the Pax5 Gene in Chicken B Cells.
  JOURNAL   PLoS One. 2015 Jan 21;10(1):e0116579. doi: 
            10.1371/journal.pone.0116579. eCollection 2015.
  PUBMED    25607658
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-21"; volume: "10(1):e0116579. doi"; pages: " 
            10.1371/journal.pone.0116579. eCollection 2015"
COMMENT     Sequence Label: 3xFNLDD/pCMV-7.1
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(181..432,433..789)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     724..745
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      121 gcgaattcat cgatagatcg atcccctcca aaaaagaaga gaaaggtcga acctgaggga
      181 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
      241 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
      301 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
      361 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
      421 cgtgtggctg ccggtgaacc acttctggcg caacagcata ttgaaggtca ttatcaggtc
      481 gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
      541 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
      601 aacggtcagg tcgttgtcgc acgtattgat gacgaggtta ccgttaagcg cctgaaaaaa
      661 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgtagat
      721 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
      781 tggctgtagt cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct
      841 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
      901 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag ggggg
//
