LOCUS       sequence-132674-        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFNLDD/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..184
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             221..241
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(254..505,506..862)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     797..818
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     primer_bind     complement(1021..1041)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
       61 aaggatccca gtgtggtggt acgggaattc gtttagtgaa ccgtcagaat taaccatgga
      121 ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga
      181 caagcttgcg gccgcgaatt catcgataga tcgatcccct ccaaaaaaga agagaaaggt
      241 cgaacctgag ggaatgaaag cgttaacggc caggcaacaa gaggtgtttg atctcatccg
      301 tgatcacatc agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg
      361 gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat
      421 tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg caggaagagg aagaagggtt
      481 gccgctggta ggtcgtgtgg ctgccggtga accacttctg gcgcaacagc atattgaagg
      541 tcattatcag gtcgatcctt ccttattcaa gccgaatgct gatttcctgc tgcgcgtcag
      601 cgggatgtcg atgaaagata tcggcattat ggatggtgac ttgctggcag tgcataaaac
      661 tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt gatgacgagg ttaccgttaa
      721 gcgcctgaaa aaacagggca ataaagtcga actgttgcca gaaaatagcg agtttaaacc
      781 aattgtcgta gatcttcgtc agcagagctt caccattgaa gggctggcgg ttggggttat
      841 tcgcaacggc gactggctgt agtcgaaatt ccagctgagc gccggtcgct accattacca
      901 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
      961 aaggaaatcc attatgtact atttaaactc gagcggccgc cagcacagtg gtcgacctgt
     1021 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1081 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtcccc
//
