LOCUS       sequence-132675-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFNLDD/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..685
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(86..105)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     582..602
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
       61 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
      121 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
      181 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
      241 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
      301 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
      361 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
      421 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
      481 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
      541 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
      601 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
      661 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
      721 gagcgcacga ccccatgcat cgtcgacgat aaaataaaag attttattta gtctccagaa
      781 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
      841 ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac
      901 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      961 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     1021 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
     1081 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga a
//
