LOCUS       sequence-132676-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.
            A retroviral expression vector with the puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             173..238
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      254..263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             263..304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             317..337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
       61 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
      121 cccagtgtgg tggtacggga attcgtttag tgaaccgtca gaattaacca tggactacaa
      181 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct
      241 tgcggccggc cgcgccacca tgggaaaacc tattcctaat cctctgctgg gcctggattc
      301 taccggaggc atggccccta agaaaaagcg gaaggtggac ggcggagtgg acctgagaac
      361 actgggatat tctcagcagc agcaggagaa gatcaagccc aaggtgagat ccacagtggc
      421 ccagcaccac gaagccctgg tgggacacgg atttacacac gcccacattg tggccctgtc
      481 tcagcaccct gccgccctgg gaacagtggc cgtgaaatat caggatatga ttgccgccct
      541 gcctgaggcc acacacgaag ccattgtggg agtgggaaaa cagtggtctg gagccagagc
      601 cctggaagcc ctgctgacag tggccggaga actgagagga cctcctctgc agctggatac
      661 aggacagctg ctgaagattg ccaaaagggg cggagtgacc gcggtggaag ccgtgcacgc
      721 ctggagaaat gccctgacag gagcccctct gaacctgacc cccgaacagg tggtggccat
      781 tgccagcaac atcggcggca agcaggccct ggaaaccgtg cagagactgc tgcccgtgct
      841 gtgccaggcc catggcctga cacctgaaca ggtggtggct atcgcctcta acaacggagg
      901 aaaacaggct ctggaaacag tgcagcggct gctgcctgtg ctgtgtcagg ctcacggctt
      961 gactccagaa caggtggtgg ctattgcttc caacaacggg gggaaacagg ccctggaaac
     1021 tgtgcagcgc ctgctgccag tgctgtgcca ggctcacgga ctgacccc
//
