LOCUS       sequence-13268-e        7990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Express-Kan SMR5917
FEATURES             Location/Qualifiers
     source          1..7990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2275..3090)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIXXK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHXXLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2998..3017
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3082..3101
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3411..3695
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3618..3637
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(6011..6028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6182..6770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6262..6281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6941..7732,7733..7801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7564..7583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7802..7906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7974..7990,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctggcgc gcctaggcca tatgggcccg ggcgagcata ttgatccgct gcaaactgaa
       61 attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg
      121 agctgccagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc
      181 tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc aaatggcgga agtaccaacc
      241 tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt
      301 acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctctat
      361 atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt gattaacttc
      421 aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt
      481 gtcggatgcg atgcttgcgc atcttatccg acctacgaat cgcatcgaat ctgtaggcca
      541 gataaggcat tttcgcagca tccggcactt attgtcggat gcgatgcttg cgcatcttat
      601 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
      661 atagttaatt gctcttactt cttcgcctct gcaaccactt tactacccac gccgcggtta
      721 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta
      781 tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac
      841 ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
      901 gtattggcat caacatccag cgagcggccc agttgcgcca caccatcacc gaaagcattg
      961 aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac
     1021 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
     1081 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
     1141 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
     1201 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc atcagtacgc
     1261 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
     1321 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagagca gagaacaccg
     1381 atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc
     1441 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gccaccatca
     1501 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
     1561 cccggagcgg cagggacata taccgttccc tgatactcac caggcatcac ggcaatatac
     1621 tggcgcttgt tggtacgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
     1681 gttacacctt gagtgcccgc cgggccgaca acaaagtcag gttgcgcagg cagggtaatc
     1741 ggggaaggat tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg
     1801 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcggtttga
     1861 tcagaaggac gttgatcggg cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc
     1921 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
     1981 agaaaaatga atccgttgaa ggcatgtctg agctcctgtc atgcatgcaa gcttcctgaa
     2041 tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg
     2101 tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa
     2161 gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccgccgtc
     2221 ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa
     2281 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     2341 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     2401 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2461 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     2521 cggtgagaat ggcaannnnn natgcatttc tttccagact tgttcaacag gccagccatt
     2581 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     2641 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     2701 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     2761 taatacctgg aatgctgttt tnnnnnngat cgcagtggtg agtaaccatg catcatcagg
     2821 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     2881 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2941 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     3001 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     3061 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     3121 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     3181 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     3241 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     3301 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     3361 tggggcgatt caggaagctt gcatgacagg agctctcccg ccattcagag aagaaaccaa
     3421 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
     3481 ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
     3541 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
     3601 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
     3661 acgcttttta tcgcaactct ctactgtttc tccatacccg ttttaggagg tactgagcat
     3721 gcgtctagag ctcgaggtag atctgaaagc ttgaatcgat gtacgtacta taccggtgcg
     3781 aaacgggaag gtaaaaagac aaaaagttgt ttttaatacc tttaagtgat accagatggc
     3841 attgcgccat ctggcagagt gattaactaa acatcgcagt aatcgaggcg cttgccagag
     3901 agtggaaatg aacgttaaac ccgaccatcg cgccgctggc accttcatcg acatcaatac
     3961 gttctatatc cagcgcgtga acggtaaaaa tgtagcgatg agtttcgcct ttcggcggtg
     4021 ctgcgccatc gtacccggtt ttaccaaagt cggtacgcgt ctgcaaaacg ccgtctggca
     4081 ttgctaccag accagagcca aacccttgcg gtaatacgcg ggtatcagcg ggtaagttaa
     4141 caactaccca gtgccaccag ccggagccgg ttggcgcatc cgggtcgtag caggtgacaa
     4201 caaaactttt cgttcccgca ggaacatcat cccacgccag atgcggtgaa atattatcgc
     4261 catcgtaacc catgccgtta aagacatgac gatgcggcaa tttatcgcca tcgcgcagat
     4321 cgttactgat gagtttcatg aaccctcctt tcttgtttgc agaaagtgta gccagaaacc
     4381 ctcacgcgga cttctcgtta ttggcaaaaa aatgtttcat cctgtaccgc gcggttaacc
     4441 gctgcggtca gacgctgcaa ctgttgcggg agaataatat agggcggcat caggtaaatc
     4501 agtttgccaa aaggccggat ccagacaccc tgttcgacaa agaatttttg cagcgccgcc
     4561 atattcaccg gatgagtggt ttcgaccacg ccaatggccc ccagtacgcg cacatcggca
     4621 accatttcgg catcacgggc gggggcaagt tgctcgcgca gctgtacttc aatatccgcc
     4681 acctgttgct gccagtcgcc agattcgaga atcgccaggc tggcgtttgc tgccgcgcag
     4741 gccagcggat tgcccataaa agttggccca tgcataaagc aaccggcttc accgttactg
     4801 atggtttctg caacctcgcg cgtggtgagt gtggcggaaa gggtcattgt gccgccggtt
     4861 aaggctttac cgaggcacaa aatgtccggc gcgatttctg catgttcaca ggcaaacagt
     4921 ttcccggtac gaccaaatcc agtggcgatc tcgtcggcaa tcagcaagat accttcgcga
     4981 tcgcatattt tgcggattcg ttttaaccat tccggatggt acatgcgcat cccgcctgcg
     5041 ccctggacaa tcggctcaat gatcaccgcc gcgatttcat gacgatgcgc cgccatcagg
     5101 cgggcaaagc ccaccatatc gcgctcatcc cattcgccat ccatgcggct ttgcggggcg
     5161 ggagcaaaca ggttttctgg caggtagcct ttccacagac tgtgcattga gttatccgga
     5221 tcgcacaccg acatcgcgcc aaaggtatcg ccatgataac cattgcggaa ggtcagaaaa
     5281 cgctggcgcg cttcgccttt ggcttgccag tactgcaacg ccattttcat cgccacttcc
     5341 accgctacgg aaccggagtc cgcgagaaaa acgcactcca gcggttgcgg cgtcatcgcc
     5401 accagtttgc ggcacagctc aatggctggc gcatgggtga taccgccaaa catcacatgc
     5461 gacatggcat caatttgcga cttcatcgcc gcattaagct gcgggtgatt gtagccgtgg
     5521 atcgccgccc accaggacga cataccgtca accaggcgtc tgccgtcaga caaaatcagc
     5581 tcgcaacctt cggcgctcac caccggataa accggcagag gggaggtcat ggatgtgtat
     5641 gggtgccaga tatggcgttg gtcaaaggca agatcgtccg ttgtcataat cgacttgtaa
     5701 accaaattga aaagatttag gtttacaagt ctacaccgaa ttaacaacaa aaaacacgtt
     5761 ttggagaagc cccatggctc accgcccacg ctggacattg tcgcaagtca cagaattatt
     5821 tgaaaaaccg ttgctggatc tgctgtttga agcgcagcag gtgcatcgcc agcatttcga
     5881 tcctcgtcag gtgcaggtca gcacgttgct gcatatggcg cgcctaggcc aattgggccc
     5941 gggcatatgg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     6001 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6061 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     7261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     7561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7981 gtatcacgag
//
